Development of PCR Technology for Identification of the Restriction Fragment Length Polymorphism(RFLP) of the Immunoglobulin Allotypes in Periodontal Patients.
10.5051/jkape.1999.29.2.349
- Author:
Jeom Il CHOI
1
;
Sung Jo KIM
;
In Hoo KIM
Author Information
1. Department of Periodontology, School of Dentistry, Pusan National University, Korea.
- Publication Type:Original Article
- Keywords:
Immunoglobulin Allotype;
Restriction Fragment Length Polymorphism;
Polymerase Chain Reaction
- MeSH:
DNA;
Genetics, Population;
Humans;
Immunoglobulin Allotypes*;
Immunoglobulins*;
Lymphocytes;
Polymerase Chain Reaction*;
Polymorphism, Restriction Fragment Length
- From:The Journal of the Korean Academy of Periodontology
1999;29(2):349-354
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.
