Suppression of nitric oxide and interleukin-6 production by methanol extract of Sophorae Flos in macrophage cells.
10.5051/jkape.2005.35.1.9
- Author:
Ji Eun LEE
1
;
Ju Youn LEE
;
Jeom Il CHOI
;
Chong Kwan KIM
;
Sung Jo KIM
Author Information
1. Department of Periodontology, College of Dentistry, Pusan National University, Korea.
- Publication Type:Original Article
- Keywords:
Sophorae Flos;
Prevotella intermedia LPS;
RAW264.7 cells;
nitric oxide;
interleukin-6
- MeSH:
Animals;
Anti-Inflammatory Agents;
Blotting, Western;
Enzyme-Linked Immunosorbent Assay;
Interleukin-6*;
Macrophages*;
Methanol*;
Mice;
Nitric Oxide*;
Periodontal Diseases;
Periodontitis;
Prevotella intermedia;
Reverse Transcription;
RNA, Messenger;
Sophora*
- From:The Journal of the Korean Academy of Periodontology
2005;35(1):9-19
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Both nitric oxide (NO) and interleukin-6 (IL-6) have been thought to have a role in the pathogenesis of inflammatory periodontal disease as it does in other inflammatory diseases, and the inhibitors of NO and IL-6 production have been considered as potential anti-inflammatory agents. In this study, we evaluated methanol extract of Sophorae Flos for inhibition of NO and IL-6 production in Prevotella intermedia LPS-induced mouse macrophages RAW264.7 cells. Dried Sophorae Flos was sliced, and extracted with 100% methanol. LPS from P. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supernatants and IL-6 was measured using mouse IL-6 ELISA kit. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. The methanol extract of Sophorae Flos concentration-dependently reduced the production of NO and the expression of iNOS protein and mRNA in RAW264.7 cells treated with P. intermedia LPS. Sophorae Flos also suppressed IL-6 production and the expression of IL-6 mRNA in RAW264.7 cells stimulated by P. intermedia LPS. The inhibition of NO and IL-6 production by Sophorae Flos may be useful in the therapy of inflammatory diseases such as periodontitis. This hypothesis, however, remains to be tested.
