Diversity of Endophytes in Panax quinquefolium Root
10.13422/j.cnki.syfjx.20230214
- VernacularTitle:西洋参根部内生微生物多样性分析
- Author:
Zhengpeng WANG
1
;
Tianrui LIU
2
;
Yuyang ZHAO
3
;
Yan JIN
3
;
Luqi HUANG
1
;
Yuan YUAN
3
Author Information
1. School of Pharmacy， Jiangsu University， Zhenjiang 212013， China
2. Institute of Traditional Chinese Medicine Health Industry， China Academy of Chinese Medical Sciences， Nanchang 330115， China
3. National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
- Publication Type:Journal Article
- Keywords:
Panax quinquefolium;
endophyte;
16S sequencing;
internal transcribed spacer （ITS） sequencing;
diversity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(7):160-167
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the community structure of endophytes in Panax quinquefolium root and explore the dominant endophytic bacteria and fungi， to provide scientific basis for the establishment of endophytic microbial bank in P. quinquefolium root. MethodInternal Transcribed Spacer （ITS） sequencing and 16S sequencing were performed on six P. quinquefolium root samples collected from Wendeng， Shandong province on PacBio Sequel Ⅱ. ResultA total of 8 phyla， 11 classes， 23 orders， 27 families and 53 genera of endophytic bacteria were identified in P. quinquefolium root， among which an unidentified Burkholderiaceae and an unidentified Rhizobiaceae were dominant. A total of 9 phyla， 23 classes， 35 orders， 43 families and 48 genera of endophytic fungi were identified in P. quinquefolium root， among which an unclassified Helotiales and Pseudogymnoascus were dominant. The community structure of endophytic bacteria revealed that the roots were selectively enriched with nitrogen-fixing bacteria such as unidentified Rhizobiaceae， Bradyrhizobium and Herbaspirillum， which suggested that nitrogen is important for the growth of P. quinquefolium root. The community structure of endophytic fungi indicated that P. quinquefolium in Shandong province might be infected by unclassified Helotiales. ConclusionThere is a rich diversity of endophytic bacteria and fungi in P. quinquefolium root， which provides scientific basis for studying the interaction of the plant with endophytic microorganisms and screening the endophytes to promote the growth of P. quinquefolium root.
