Puerarin Inhibits Inductible Nitric Oxide Synthase expression in Lipopolysaccharide -induced RAW264.7 Macrophage Cells
- VernacularTitle:葛根素抑制脂多糖诱导RAW264.7巨噬细胞表达诱导型一氧化氮合酶
- Author:
Jian YU
;
Wenzhi HU
;
Ling JI
;
Lie SUN
- Publication Type:Journal Article
- Keywords:
Puerarin, macrophage cells, lipopolysaccharide, inductible nitric oxide synthase
- From:
Chinese Journal of Rehabilitation Theory and Practice
2010;16(9):835-836
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of Puerarin on expression of inductible nitric oxide synthase (iNOS) in RAW264.7 macrophage cells induced by lipopolysaccharide （LPS）. MethodsThe protein and mRNA levels of lipopolysaccharide (LPS)-induced iNOS was determined in RAW264.7 macrophage cells using Western blotting and RT-PCR respectively. ResultsPuerarin inhibited expression of iNOS concentration dependently (P<0.01). ConclusionPuerarin may reduce iNOS level, result in prevention of inflammation.
