Constructing a Completely Biological Hybrid Scaffold for Small-Diameter Vascular Tissue Engineering Using Fibrin and Decellularized Artery
- VernacularTitle:利用纤维蛋白胶复合血管脱细胞基质制备全生物型小口径组织工程血管支架材料的研究
- Author:
Guofeng LIU
;
Zhijuan HE
;
Daping YANG
;
Xuewu XU
;
Lihong REN
;
Ying LIU
;
Qingchun LI
- Publication Type:Journal Article
- Keywords:
vascular grafts, tissue engineering, biological scaffold, fibrin, extracellular matrix, biomechanical
- From:
Chinese Journal of Rehabilitation Theory and Practice
2010;16(8):748-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a completely biological hybrid scaffold for small-diameter vascular tissue engineering using porcine fibrin and decellularized canine carotid artery.MethodsPorcine fibrin was sprayed coating on the external surface of decellularized canine carotid artery to construct completely biological hybrid scaffold for small-diameter vascular tissue engineering. The completely biological hybrid scaffold was evaluated with Hematoxylin and Eosin (H&E) staining, scanning electron microscopy and biomechanics test.ResultsHistology examination revealed that the porcine fibrin was sprayed coating uniformly on the external surface of decellularized canine carotid artery. Scanning electron microscopy examination confirmed that the external surface of completely biological hybrid scaffold was smooth and uniformly. Compared with fresh canine carotid artery and decellularized artery, the biological hybrid scaffold had similar burst and breaking strength. Furthermore, compared with decellularized artery, the biological hybrid scaffold had higher compliance.ConclusionThe porcine fibrin was sprayed coating uniformly on the external surface of decellularized canine carotid artery to prepare a completely biological hybrid scaffold for small-diameter vascular tissue engineering. The biological hybrid scaffold had appropriate biomechanical properties and had potential to serve as scaffolds for small-diameter vascular tissue engineering.
