In Vitro Antimicrobial Activity and Aggregation Abilities of Probiotic Lactobacillus casei and Lactobacillus salivarius Against Oral Pathogens

Darshyna Theena Thayalan; Rosmaliza Abdullah; Siti Suraiya Md Noor; Suharni Mohamad

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In Vitro Antimicrobial Activity and Aggregation Abilities of Probiotic Lactobacillus casei and Lactobacillus salivarius Against Oral Pathogens

Author: Darshyna Theena Thayalan ¹ ; Rosmaliza Abdullah ² ; Siti Suraiya Md Noor ² ; Suharni Mohamad ¹
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1. School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
2. Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
Publication Type:Journal Article
Keywords: Agar diffusion; Auto-aggregation; Coaggregation; Lactobacillus spp.; Oral pathogens
MeSH: Lacticaseibacillus casei--pathogenicity; Ligilactobacillus salivarius--pathogenicity
From:Archives of Orofacial Sciences 2021;16(2):153-163
CountryMalaysia
Language:English
Abstract: ABSTRACT:The antagonistic effect of probiotics against oral pathogens merits exploration because these bacteria are beneficial to the host’s health. The antimicrobial activity of two probiotic strains, Lactobacillus casei and Lactobacillus salivarius, as well as L. casei and L. salivarius combination (1:1), was investigated against Streptococcus mutans, Streptococcus sobrinus, Candida albicans, Candida glabrata and Candida tropicalis using agar-well diffusion, auto-aggregation and coaggregation assays. L. salivarius cell-free supernatant (CFS) alone exhibited greater inhibitory effect against Streptococci spp. compared to L. casei CFS alone and the combination. However, no inhibition was observed for Candida spp. L. salivarius alone exhibited significantly stronger auto-aggregation than L. casei alone (p ≤ 0.05) and L. casei and L. salivarius combination. L. salivarius exhibited strong coaggregation ability with Candida spp., followed by Streptococci spp. while L. casei exhibited coaggregation only with Streptococci spp. However, L. casei and L. salivarius combination did not display any coaggregation with all strains. L. salivarius alone exhibited a stronger antagonistic effect on the tested organisms than L. casei alone or in combination. Based on the results, both probiotic strains showed good antimicrobial activities against oral pathogens and should be further studied for their human health benefits.
Full text:2.2021my0015.pdf