Identification of Newcastle Disease Virus sub-genotype VII 1.1 isolated from chickens in Sabah, Malaysia
https://doi.org/10.47665/tb.39.4.015
- Author:
Syamsiah Aini, S.
1
;
Leow, B.L.
1
;
Faizul Fikri, M.Y.
1
;
Muhammad Redzwan, S.
1
;
Faizah Hanim, M.S.
1
Author Information
1. Veterinary Research Institute, Ipoh, Perak, Malaysia
- Publication Type:Journal Article
- Keywords:
Newcastle Disease Virus (NDV);
VII.1.1;
chickens;
Sabah;
Malaysia
- From:Tropical Biomedicine
2022;39(No.4):579-586
- CountryMalaysia
- Language:English
-
Abstract:
Newcastle disease (ND) is an extremely contagious and fatal viral disease causing huge economic losses
to the poultry industry. Following recent ND outbreaks in Sabah in commercial poultry and backyard
farms, it was speculated that this could be due to a new introduction of Newcastle Disease Virus (NDV)
genotype/sub-genotype. Here we report the genetic characterization of NDVs isolated from Sabah during
early 2021. All isolates were amplified and sequenced with primers specific to the viral fusion (F) gene
using reverse transcription-polymerase chain reaction (RT-PCR). Nucleotide sequence analysis of the F
gene showed that all isolates shared similar homology of 99.4% with NDV strain from Iran isolated in
2018. Amino acid sequences of the F protein cleavage site revealed the motif of 112RRQKRF117 indicating
all isolates were of virulent strain. Phylogenetic analysis demonstrated that all isolates were clustered
under sub-genotype VII 1.1 and clustered together with isolates from Iran (previously known as subgenotype VIIl). The present findings suggested that there is an emerging of a new sub-genotype into
the poultry population in Sabah and this sub-genotype has never been reported before in Malaysia.
Therefore, transboundary monitoring and continuous surveillance should be implemented for proper
control and prevention of the disease. A further molecular epidemiological analysis of NDV is needed
to well understand the circulatory patterns of virulent strains of NDV in the country to prevent future
outbreaks.
- Full text:8.2022my1380.pdf
