Detection of pathogenic Vibrio species and antibiogram activity in Asian Seabass (Lates calcarifer) in Tumpat, Kelantan
https://doi.org/10.47665/tb.39.4.013
- Author:
Auzureen, A.M.Z.1
1
;
Michael, M.S.
1
;
Mohamed, M.
1
;
Peng, T.L.
1
;
Fauzi, F.
1
;
Mohamad, N.F.A.
1
;
Ahmad, N.S.
1
;
C.W. Salma, C.W.Z.
1
;
Hamdan, R.H.
1
Author Information
1. Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa 16100 Kota Bharu, Kelantan, Malaysia
- Publication Type:Journal Article
- Keywords:
Vibrio spp.;
Lates calcarifer;
antibiotic susceptibility test;
multiple antibiotic resistance (MAR)
- From:Tropical Biomedicine
2022;39(No.4):569-574
- CountryMalaysia
- Language:English
-
Abstract:
Some of Vibrio species is well known as pathogenic bacteria in aquaculture and the marine industry. Its
infection is able to generate a massive outbreak and affect the fish population, especially for net caged
fish such as seabass. This study was conducted to investigate the prevalence of Vibrio spp. isolated
from seabass (Lates calcarifer) in Sri Tujuh Lagoon, Tumpat, Kelantan. Then, to determine the antibiotic
resistance in Vibrio isolates. Polymerase chain reaction (PCR) was used to detect Vibrio species using
specific primer VR169 and VR744 with estimation base pair size band, 597 bp and further identified
by sequencing. On the other hand, antibiotic susceptibility tests were continued by using 13 types of
antibiotics; kanamycin (K30), chloramphenicol (C30), neomycin (N10), ampicillin (AMP10), nitrofurantoin
(F300), tetracycline (TE30), streptomycin (S10), norfloxacin (NOR10), ciprofloxacin (CIP5), nalidixic acid
(NA30), gentamicin (CN10), doxycycline (DO30) and sulfamethoxazole (SXT100). As a result, 14 Vibrio
isolates were identified, including Vibrio fluvialis (n=6), Vibrio parahaemolyticus (n=3), Vibrio harveyi
(n=2) and each isolate for Vibrio vulnificus, Vibrio alginolyticus and Vibrio spp. The results showed that
all isolates were sensitive to most antibiotics except ampicillin, neomycin and streptomycin. The MAR
index value was ranging from 0 to 0.31. This study demonstrates the prevalence of Vibrio spp. in seabass
and the report on multidrug resistance strains that could be of concern to the fish farmers. In addition,
data from this study can be further used in fish disease management plans.
- Full text:8.2022my1378.pdf
