Effects of artemisinin and hydroxychloroquine on cytokines in experimental sepsis
https://doi.org/10.47665/tb.39.4.010
- Author:
Büyükcavlak, M.
1
;
Duman, I.
2
;
Eryavuz, O.D.
3
,
4
;
Ünlü, A.
3
,
4
;
Duman, A.
4
,
5
Author Information
1. University of Health Sciences, Konya City Hospital, Department of Anesthesiology and Reanimation, 42020, Karatay, Konya, Turkey
2. Necmettin Erbakan University, Meram Faculty of Medicine, Department of Medical Pharmacology, 42080, Meram, Konya, Turkey
3. Selcuk University, Faculty of Medicine, Department of Medical Biochemistry, 42130, Selç
4. uklu, Konya, Turkey
5. Selcuk University, Faculty of Medicine, Department of Anesthesiology and Intensive Care, 42130, Selç
- Publication Type:Journal Article
- Keywords:
Artemisinin;
cytokine;
hydroxychloroquine;
interleukin;
sepsis
- From:Tropical Biomedicine
2022;39(No.4):547-551
- CountryMalaysia
- Language:English
-
Abstract:
Pro-and anti-inflammatory cytokines mediate the inflammatory response in sepsis. Therefore, regulation
of cytokines with medications in risky situations may protect the patients from sepsis. Hydroxychloroquine
and artemisinin are antimalarial drugs with immunomodulatory properties. In this study, we intended
to investigate the effects of artemisinin and hydroxychloroquine on the cytokines released during sepsis
in the rat model. Twenty-four rats were randomized into four groups. The control group received oral
saline, the sepsis group received oral saline and intraperitoneal lipopolysaccharide toxin (LPS), the
artemisinin-treated sepsis group received oral 33.33 mg/kg of artemisinin, and the hydroxychloroquinetreated sepsis group received oral 33.33 mg/kg of hydroxychloroquine before LPS injection. Three hours
later, serum cytokines were measured. An increase was detected in TNF-a, IL-1, and IL-6 levels in the
sepsis group compared to the control (p<0.01). Oral pretreatment with artemisinin resulted in significant
downregulation only of IL-1 levels (p<0.01). Cytokines IL-1 and IL-6 were significantly downregulated in
the serum of LPS-induced rats pretreated with oral hydroxychloroquine than rats with sepsis (p<0.01).
Decreases observed in TNF-a and IL-10 levels were insignificant. These results demonstrated that
both artemisinin and hydroxychloroquine attenuate the release of pro-inflammatory cytokines three
hours after LPS-induced sepsis in rats. A significant decrease was observed in serum IL-1 and IL-6 levels
with hydroxychloroquine and IL-1 levels with artemisinin. Based on our findings, we suggest that the
therapeutic potential of artemisinin and hydroxychloroquine may be beneficial in preventing cytokine
storm during sepsis, and further research is needed to determine the optimal timing of administration.
- Full text:8.2022my1375.pdf
