Comparison of the Effects of Single Sperm Cryopreservation and Conventional Cryopreservation on Embryo Culture and Pregnancy in Patients Undergoing Micro-TESE
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2023.0120
- VernacularTitle:单精子冷冻和常规冷冻对显微取精患者的胚胎培养及妊娠影响的比较
- Author:
Ting-ting LI
1
;
Gui-hua LIU
1
;
Jing ZHANG
1
;
Gui-hua SUN
1
Author Information
1. Reproductive Medicine Center， The Sixth Affiliated Hospital， Sun Yat-sen University，Guangzhou 510655， China
- Publication Type:Journal Article
- Keywords:
micro-TESE;
single sperm cryopreservation;
fertilization rate;
available embryo rate;
high-quality embryo rate;
clinical pregnancy rate
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2023;44(1):145-149
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo compare the effects of single sperm cryopreservation and conventional cryopreservation on embryo culture and pregnancy in patients undergoing microdissection testicular sperm extraction （micro-TESE）. MethodsA retrospective analysis was done on the patients who underwent micro-TESE at the Reproductive Medicine Center in the Sixth Affiliated Hospital of Sun Yat-Sen University between January 2018 and December 2021. The single sperm cryopreservation group included 39 patients undergoing single sperm cryopreservation and 307 MII oocytes. The conventional cryopreservation group included 39 patients undergoing conventional cryopreservation and 410 MII oocytes. Propensity score matching （PSM） was performed to balance the selection bias. The fertility rate， embryo culture and pregnancy of these two groups were compared. ResultsThere was no statistical difference in age， body mass index （BMI）， years of infertility， basal FSH， basal LH， basal E2， AMH， AFC， number of oocytes retrieved and number of transferred embryos between the two groups （P>0.05）. No significant difference was found in fertilization rate （65.8% vs. 60.49%）， available embryo rate （67.82% vs. 58.87%） and high-quality embryo rate （70.80% vs. 75.34%）. The single sperm cryopreservation group had significantly lower clinical pregnancy rate than conventional cryopreservation group （45.45% vs. 70.0%， P=0.049）. ConclusionIf the patients undergoing micro-TESE have very few sperms， single sperm cryopreservation could be an effective choice to increase the utilization of each sperm and ensure the subsequent sperm retrieval after thawing， but the clinical pregnancy rate is decreased. Due to the limited number of cases， we need a large additional number of cases to observe and analyze.
