VWF Damage Based on Novel Maglev Taylor-Couette Blood-Shearing Circulation Platform
10.16156/j.1004-7220.2022.04.19
- VernacularTitle:基于新型磁悬浮Taylor-Couette血液剪切平台研究VWF损伤规律
- Author:
Bin LU
1
;
Liudi ZHANG
1
;
Xu MEI
1
;
Chengke YIN
1
;
Qiubo JIANG
1
;
Nana HUAN
1
;
Peng WU
1
Author Information
1. Laboratory of Artificial Organ Technology, School of Mechanical and Electrical Engineering, Soochow University
- Publication Type:Journal Article
- Keywords:
blood-shearing device;
circulation loop;
von Willebrand factor (VWF);
exposure time;
shear stress
- From:
Journal of Medical Biomechanics
2022;37(4):E699-E705
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study von Willebrand factor(VWF) damage based on a novel Maglev Taylor-Couette blood-shearing device. Methods The magnetic levitation (maglev) Taylor-Couette blood-shearing device was designed, and the blood-shearing platform was built. Fresh porcine blood was tested in circulation loop for 1 hour at laminar flow state. VWF damage was assessed by analyzing sample through Western blot and enzyme-linked immunosorbent assay. Results With the increase of exposure time and shear stress, a large number of high molecular weight VWF multimers were degraded into low molecular weight VWF. The maximum rate of degradation was 569%. When the shear stress increased from 18 Pa to 55 Pa, the ratio of VWF-Rco to VWF-Ag decreased from 45.7% to 32.8%. ConclusionsCompared with initial sample, the VWF damage was mainly manifested by the decrease of high molecular weight VWF and the decrease of VWF activity, and VWF-Ag did not change significantly. The novel maglev Taylor-Couette blood-shearing device can quantitatively control the flow parameters (exposure time and shear stress), and be used for blood damage research in vitro, thus providing references for the design and optimization of extracorporeal membrane oxygenation and blood pump.
