Single Cell Traction Force Measured by Foldable Microplates
10.16156/j.1004-7220.2022.02.15
- VernacularTitle:可折叠微板测量单细胞三维状态下的牵张力
- Author:
Lijun ZHAO
1
;
Chenyan WANG
1
;
Quanyou ZHANG
2
;
Di HUANG
3
;
Jinchuan HOU
4
;
Weiy CHEN
3
Author Information
1. Shanxi Key Laboratory of Materials Strength & Structural Impact,Institute of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology
2. Research Center for Nano⁃ biomaterials and Regenerative Medicine, Institute of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology
3. Shanxi Key Laboratory of Materials Strength & Structural Impact, Research Center for Nano⁃ biomaterials and Regenerative Medicine, Institute of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology
4. College of Mathematics, Taiyuan University of Technology
- Publication Type:Journal Article
- Keywords:
cell traction force;
single cell culture;
foldable microplate;
bending moment
- From:
Journal of Medical Biomechanics
2022;37(2):E287-E291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To fabricate a foldable microplate for single cell culture and establish finite element model of the folding microplate, so as to calculate traction force of single cells during contraction in three-dimensional (3D) state.Methods The folding angle of the microplate casued by cell traction force was calculated. Then the relation between bending moment and folding angle as well as the relation between traction force and bending moment were derived by using finite element simulation, so as to realize the characterization of traction force for singel cell in 3D state.Results The folding angles of the microplate with HSF and MC3T3-E1 cells in 3D state were 73°-173° and 49°-138°, respectively. The single cell traction forces of HSF and MC3T3-E1 cells were 55-210 nN and 52-161 nN, respectively.Conclusions The proposed method for measuring traction force of single cells in 3D state by fabricating the foldable microplate for single cell culture will provide some references for further development of calculating traction forces in 3D cell adhesion, spreading and migration.
