Study on quality standard of Yi medicine Gynura japonica and quality evaluation of medicinal materials from different producing areas
- VernacularTitle:彝药菊三七的质量标准及不同产地药材的质量评价
- Author:
EQIXIAOLI
1
,
2
,
3
;
Jixiu SHEN
1
,
4
;
Jiang LUO
1
,
2
,
3
;
DIJIUCIGA
1
,
2
,
3
;
Yuan LIU
2
,
3
,
5
;
Zhengming YANG
2
,
3
,
6
;
Ying LI
1
,
2
Author Information
1. School of Pharmacy，Southwest Minzu University，Chengdu 610225，China
2. Sichuan Laboratory for Qiangyi Medical Resources Protection and Utilization Technology Engineering，Chengdu 610225，China
3. Key Laboratory for the Protection and Utilization of Ethnic Medicinal Resources in the Qinghai-Tibet Plateau，National Ethnic Affairs Commission of the People’s Republic of China，Chengdu 610225，China
4. Liangshan Yi Autonomous Prefecture Institute for Food and Drug Control，Sichuan Xichang 615000，China
5. Institute of Ethnic Medicine，Southwest Minzu University，Chengdu 610225，China
6. Qinghai-Tibet Plateau Research Institute，Southwest Minzu University，Chengdu 610225，China
- Publication Type:Journal Article
- Keywords:
Gynura japonica;
quality standard;
senecionine;
seneciphylline;
total alkaloids;
multivariate statistical analysis
- From:
China Pharmacy
2023;34(3):309-314
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To improve the quality standard of Yi medicine Gynura japonica， and to evaluate its quality. METHODS Using 15 batches of G. japonica from different producing areas as samples， the contents of water， total ash， acid- insoluble ash and water-soluble extract were determined according to the method stated in part Ⅳ of Chinese Pharmacopoeia （2020 edition）. The contents of total alkaloid （calculated by senecionine） was determined by UV spectrophotometry. The contents of senecionine and seneciphylline were determined by HPLC. Using above 7 indexes as evaluation indexes， cluster heat map analysis， principal component analysis （PCA） and entropy weight approximation ideal ranking （TOPSIS） were used to evaluate the quality of medicinal material comprehensively. RESULTS Among 15 batches of G. japonica， the moisture contents were 8.88%-12.60%， the total ash contents were 4.43%-11.02%， the acid-insoluble ash contents were 0.56%-3.45%， the water-soluble extract contents were 21.71%-53.91%， the total alkaloid contents （calculated by senecionine） were 0.15%-0.39%， and the contents of senecionine and seneciphylline were 0.01% -0.05% and 0.01%-0.06% respectively. According to the results of various indicators， it was preliminarily proposed that the water content in the sample of G. japonica should not exceed 13.00%， the total ash content should not exceed 11.50%， the acid-insoluble ash content should not exceed 3.70%， the water-soluble extract should not be less than 20.70%， the total alkaloid content should not be less than 0.15%， the contents of senecionine and seneciphylline should not be less than 0.01% both. The results of cluster heat map analysis showed that the 15 batches of samples could be divided into four categories； the results of PCA and TOPSIS showed that the samples with high-quality ranking were jsq-2， jsq-5， jsq-6 and jsq-10， and the samples with low-quality ranking were jsq-4， jsq-13 and jsq-14. CONCLUSIONS In this study， the quantitative analysis method of total alkaloids （calculated by senecionine）， senecionine and seneciphylline in G. japonica is established， and the limits of each index are preliminarily determined. Among 15 batches of samples， the qualities of medicinal material collected from Linza Village of Ganluo County of Liangshan Yi Autonomous Prefecture， Machangping Village of Luojishan Town of Puge County of Liangshan Yi Autonomous Prefecture and other places are better.
