Single Nucleotide Polymorphism at +276 g>T of the Adiponectin gene and Plasma Adiponectin Level in Myanmar Type 2 Diabetic Patients
https://doi.org/10.15605/jafes.033.02.08
- Author:
Khin Thin Yu
1
;
Kyu Kyu Maung
2
;
Aye Thida
1
;
Thein Myint
3
Author Information
1. Department of Biochemistry, University of Medicine 2, Yangon, Myanmar
2. Department of Biochemistry, University of Medicine 1, Yangon, Myanmar
3. Department of Medicine, West Yangon General Hospital, Myanmar
- Publication Type:Journal Article
- Keywords:
Adiponectin gene;
SNP
- MeSH:
Diabetes Mellitus, Type 2
- From:
Journal of the ASEAN Federation of Endocrine Societies
2018;33(2):160-164
- CountryPhilippines
- Language:English
-
Abstract:
Objective:The aim of the study was to investigate the association between single nucleotide polymorphisms (SNP) at rs 1501299 (SNP+276 G>T) of the adiponectin gene and plasma adiponectin levels in type 2 diabetes mellitus (T2DM) patients in Myanmar.
Methodology:One hundred T2DM patients and 104 non-diabetic subjects were included in this cross-sectional analytical study. Genotype frequencies were determined by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method. Plasma adiponectin level was measured by enzyme-linked immunosorbent assay (ELISA).
Result:Genotype frequencies (GG, GT, TT) of SNP+276 in diabetic patients were 39%, 48% and 13%, respectively. The GT and TT genotypes were more frequent in T2DM patients (OR 1.98, 95% CI, 1.10-3.55; p=0.02 and OR 4.07, 95% CI, 1.34-12.3; p=0.01), respectively. The T allele of SNP+276 was significantly associated with T2DM (OR 1.96, 95% CI, 1.27-3.01; p=0.002). Mean plasma adiponectin level was significantly lower than in T2DM patients (27.41±16.7 μg/mL) compared to non-diabetic subjects (37.19±26.77 μg/mL) (p=0.002)
Conclusion:SNP+276 at rs 1501299 of the adiponectin gene was associated with type 2 diabetes and low plasma adiponectin levels in this Myanmar population.
- Full text:483-Article.pdf
