Sensitivity of Human Glioblastoma Multiforme Cell Line BT325 to Antineoplastic Drugs
- VernacularTitle:人脑多形性胶质瘤细胞系BT325 对5 种抗肿瘤药物敏感性的研究
- Author:
Ming HAN
;
Fang YUAN
;
Liping DONG
;
Zhongfang SHI
;
Hui YUAN
;
Shaohua YANG
- Publication Type:Journal Article
- Keywords:
BT325 cells, antineoplastic drugs, drug sensitivity, cell-countykit-8
- From:
Chinese Journal of Rehabilitation Theory and Practice
2011;17(9):840-843
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the sensitivity of human glioblastoma multiforme cell line BT325 to 5 antineoplastic drugs, including cisplatin (DDP), teniposide (VM26), nimustine (ACNU), temozolomide (TMZ) and vincristine (VCR). Methods BT325 cells were incubated in DMEM with 10% or 20% fetal bovine serum (FBS) or without FBS respectively. The cell numbers were counted at 24 h, 48 h, 72 h,96 h, 120 h, and 144 h, then platting and growth curve were drafted. Cell counting kite-8 was used to detect the influence of 5 drugs with different concentrations on human glioma cell line BT325. Results DDP and VM26 significantly suppressed BT325 cells(>75%) viability in a dose-dependent manner, while VCR inhibited BT325 cells (50%) growth without dose-effect relationship. In contrast, ACNU and TMZwere not effective on the viability of BT325 cells. Conclusion BT325 cells were very sensitive to chemotherapeutic drugs DDP amd VM26.
