Effects and related mechanisms of histone deacetylase inhibitors on the proliferation of choroidal melanoma C918 cell line
10.3980/j.issn.1672-5123.2023.2.02
- VernacularTitle:HDAC抑制剂对脉络膜黑色素瘤细胞系C918细胞增殖的影响及相关机制
- Author:
Yi-Meng ZHANG
1
,
2
;
Han-Yi YANG
1
,
2
;
Jia-Yi NING
1
,
2
;
Xiao-Long YAN
1
,
2
;
Jing HAN
1
,
2
Author Information
1. Xi'an Medical University, Xi'an 710068, Shaanxi Province, China
2. Department of Ophthalmology
- Publication Type:Journal Article
- Keywords:
suberoylanilide hydroxamic acid;
histone deacetylase(HDAC);
fibroblast growth factor 18;
cell proliferation;
cell cycle
- From:
International Eye Science
2023;23(2):193-197
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To elucidate the effect of histone deacetylase(HDAC)inhibitor suberoylanilide hydroxamic acid(SAHA)on the proliferation of choroidal melanoma(CM)cell line C918 and to explore the related mechanism.METHODS: Inverted fluorescence microscope was used to observe the effect of different concentrations of SAHA(0.625, 1.25 or 2.5 μmol/L)on the morphology of C918 cell. The cell viability was detected by cholecystokinin octapeptide(CCK-8)assay. Plate clone formation assay and EdU staining were carried out to measure the effect of SAHA on the cell proliferation. Meanwhile, the expressions of cell proliferation-related proteins including c-Myc, CyclinA2 and CDK2, and histone deacetylase 7(HDAC7)and fibroblast growth factor 18(FGF18)were detected by Western blot.RESULTS: Compared with the control group, the cell density was reduced in SAHA. SAHA could also promote cell shrinkage, and the inhibition on cell was in a concentration-dependent manner. CCK-8 assay showed that SAHA treatment decreased cell viability in a dose-dependent manner and the inhibition rate was 80% when SAHA at 2.5 μmol/L. Compared with the control group, Western blot showed that SAHA could suppress the expression of cell proliferation proteins including c-Myc, CyclinA2 and CDK2 in a dose-dependent manner. In addition, 1.25 μmol/L SAHA significantly decreased the numbers of EdU staining positive cells and cell clones. More importantly, SAHA could dose-dependently decrease the expression of HDAC7 and FGF18 compared with control group.CONCLUSION: SAHA could inhibit the proliferation of CM cell line C918 by inhibiting the HDAC7/FGF18 signaling pathway.
