Identification and Expression Analysis of AP2/ERF Family in Stress Responses of Pinellia ternata Based on Transcriptome Data
10.13422/j.cnki.syfjx.20230114
- VernacularTitle:基于转录组数据的半夏AP2/ERF基因家族鉴定及逆境响应分析
- Author:
Jinrong YANG
1
;
Wanning CUI
1
;
Yu ZHANG
1
;
Chen BO
1
;
Qiujie CHAO
1
;
Yanfang ZHU
1
;
Yongbo DUAN
1
;
Tao XUE
1
;
Aimin ZHANG
1
Author Information
1. Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants， College of Life Sciences， Huaibei Normal University， Huaibei 235000， China
- Publication Type:Journal Article
- Keywords:
Pinellia ternata;
AP2/ERF gene family;
gene identification;
expression analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(5):176-184
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify the functions of the AP2/ERF family members in Pinellia ternata and promote the genetic improvement of P. ternata varieties. MethodWe identified and conducted a systematic bioinformatics analysis of the AP2/ERF family member genes in P. ternata based on the three generations of transcriptome data. Real-time polymerase Chain reaction （Real-time） PCR was employed to determine the expression pattern of AP2/ERF genes in different tissues and under different stress conditions. ResultA total of eight full-length AP2/ERF family members were identified from the transcriptome data， which were classified into three sub-gene families： AP2， ERF， and DREB. The deduced AP2/ERF proteins in P. ternata had the length of 251-512 aa， the theoretical pI of 5.29-11.72， the instability index of 45.90-82.41， subcellular localization in the nucleus， and conserved domains and motifs. AP2/ERF genes were expressed in different tissues of P. ternata， with high expression levels in the leaf. The stress response experiments showed that PtERF1 mainly responded to NaCl stress. The expression of PtERF2 and PtERF4 was significantly up-regulated under low temperature and polyethylene glycol （PEG）-simulated stress. PtERF3 responded to both low temperature and NaCl stress. The expression of PtERF5 was induced by high temperature， low temperature， NaCl and PEG stress. The expression of PtERF7 was up-regulated under high temperature， while that of PtERF8 under low temperature. ConclusionThe AP2/ERF genes in P. ternata can respond to stress and have the potential functions of regulating photosynthesis and improving root stress resistance.
