Effect of Astragaloside Ⅳ on Proliferation， Migration， and Invasion of Colorectal Cancer HCT116 Cells
10.13422/j.cnki.syfjx.20221728
- VernacularTitle:黄芪甲苷对结直肠癌HCT116细胞增殖、迁移及侵袭的影响
- Author:
Benchao HOU
1
;
Zhijian HE
2
;
Haiyun LIU
3
;
Qianxia LIN
3
;
Yongqing FANG
3
;
Shimeng ZHAN
3
Author Information
1. The First Affiliated Hospital of Nanchang University， Nanchang 330006， China
2. Jiangxi Cancer Hospital， Nanchang 330029， China
3. School of Chinese Medicine， Jiangxi University of Chinese Medicine， Nanchang 330004， China
- Publication Type:Journal Article
- Keywords:
astragaloside Ⅳ;
colorectal cancer;
proliferation;
migration;
invasion
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(5):144-149
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate effect of astragaloside Ⅳ on the proliferation， migration， and invasion of colorectal cancer HCT116 cells and the underlying molecular mechanism. MethodColorectal cancer HCT116 cells were classified into blank group （DMSO） and low-dose （15.7 mg·L-1）， medium-dose （31.4 mg·L-1）， and high-dose （62.8 mg·L-1） astragaloside Ⅳ groups. After drug treatment， the morphological changes of HCT116 cells were observed under an inverted microscope. Cell viability was detected by cell counting kit-8 （CCK-8） assay， and the migration and invasion of cells were detected based on scratch assay and Transwell assay. The expression of cyclin-dependent kinase inhibitor （p21）， CyclinD1， B-cell lymphoma-2 （Bcl-2）， and Bcl-2-associated X protein （Bax） in the cells was examined by Western blot. ResultCompared with the blank group， cells in the three astragaloside Ⅳ groups demonstrated slow growth， low density， inconsistent morphology， nuclear shrinkage， degradation of cytoplasm， and high death rate. Moreover， cell viability decreased in a concentration-dependent manner in the astragaloside Ⅳ groups. Cell migration and invasion were inhibited （P<0.05， P<0.01）， and the inhibition rate was in positive correlation with the concentration of the astragaloside Ⅳ. The expression of pro-apoptotic protein Bax in low-dose， medium-dose and high-dose astragaloside Ⅳ groups increased gradually in a concentration-dependent manner， while the expression of p21， CyclinD1 and anti-apoptotic protein Bcl-2 decreased gradually in a concentration-dependent manner compared with those in the blank group （P<0.05， P<0.01）. ConclusionAstragaloside Ⅳ can suppress the proliferation， migration， and invasion of colorectal cancer HCT116 cells and promote the apoptosis， thus inhibiting the occurrence and development of colorectal cancer.
