Rutin Promotes Browning of 3T3-L1 Preadipocytes and Its Mechanism
10.13422/j.cnki.syfjx.202201727
- VernacularTitle:芦丁促进3T3-L1前脂肪细胞棕色化及其机制
- Author:
Yinglan LYU
1
;
Long CHENG
1
;
Lu SHI
1
;
Hongyu DAI
1
;
Yuhui DUAN
1
;
Yongcheng AN
2
;
Huimin LI
1
;
Chen WANG
2
;
Changhao HE
1
;
Yan HUANG
2
;
Huilin ZHANG
1
;
Wanxin FU
2
;
Yanyan MENG
3
;
Baosheng ZHAO
3
Author Information
1. College of Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 102488， China
2. College of Life Sciences， Beijing University of Chinese Medicine， Beijing 102488， China
3. Beijing Research Institute of Chinese Medicine，Beijing University of Chinese Medicine，Beijing 100029，China
- Publication Type:Journal Article
- Keywords:
rutin;
3T3-L1 preadipocytes;
adipocyte browning;
obesity;
mitochondrial biogenesis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(5):137-143
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of rutin on the browning of 3T3-L1 preadipocytes and the mechanism. MethodCell counting kit-8 （CCK-8） assay was used to detect the effect of different concentration of rutin （3.125， 6.25， 12.5， 25， 50， 100， 200 μmol·L-1） on 3T3-L1 cell activity， and Western blot to examine the effect of rutin （12.5， 25， 50 μmol·L-1） on the expression of thermogenesis-associated proteins uncoupling protein 1 （UCP1）， PR domain containing 16 （PRDM16） and peroxisome proliferator-activated receptor γ coactivator-1α （PGC-1α） in adipocytes. After the optimal concentration of rutin was determined， the effect of rutin on lipid droplet formation in adipocytes was observed based on oil red O staining， and the expression of nuclear respiratory factor 1 （NRF1）， nuclear respiratory factor 2 （NRF2） and mitochondrial transcription factor A （TFAM）， which were the landmark proteins of mitochondrial biosynthesis， was detected by Western blot. ResultCompared with the blank group， 200 μmol·L-1 rutin inhibited 3T3-L1 cell activity （P<0.01）. Compared with the blank group， at the concentration of 12.5， 25， 50 μmol·L-1 rutin significantly promoted the expression of thermogenesis-associated proteins （UCP1， PRDM16， and PGC-1α） （P<0.01）， which was determined as the optimal concentration. Compared with the blank group， 50 μmol·L-1 rutin significantly increased the immunofluorescence intensity of mitochondrial UCP1 protein in 3T3-L1 cells （P<0.01） and the expression of the markers of mitochondrial biosynthesis （NRF1， NRF2， and TFAM） （P<0.01）. In addition， 50 μmol·L-1 rutin significantly inhibited lipid droplet formation of 3T3-L1 adipocytes （P<0.01）. ConclusionRutin inhibited lipid droplet deposition in 3T3-L1 adipocytes and increased the expression of thermogenesis-related proteins （UCP1， PRDM16， and PGC-1α） and markers of mitochondrial biosynthesis （NRF1， NRF2， and TFAM）， thereby inducing the browning of 3T3-L1 adipocytes. This lays a basis for the development of drugs that safely regulate the browning of white cells.
