Babaodan Alleviates APAP-induced Acute Liver Injury in Mice by Inhibiting NLRP3/Caspase-1 Pathway
10.13422/j.cnki.syfjx.20222001
- VernacularTitle:八宝丹通过抑制NLRP3/Caspase-1通路减轻对乙酰氨基酚诱导的小鼠急性肝损伤
- Author:
Ruowei ZHAO
1
;
Qing ZHANG
1
;
Mingxing ZHU
2
;
Yueyang LIU
3
;
Zaixing CHENG
1
;
Mingqing HUANG
1
;
Yanfang ZHENG
1
;
Yanxiang LIN
1
Author Information
1. College of Pharmacy,Fujian University of Traditional Chinese Medicine(TCM),Fuzhou 350122,China
2. College of Traditional Chinese Medicine,Fujian University of TCM,Fuzhou 350122,China
3. College of Acupuncture and Moxibustion,Fujian University of TCM,Fuzhou 350122,China
- Publication Type:Journal Article
- Keywords:
Babaodan;
acute liver injury;
inflammatory factors;
NOD-like receptor pyrin domain containing 3 （NLRP3）;
cysteine aspartate-specific protease-3 （Caspase-1）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(5):122-128
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of Babaodan （BBD） on the NOD-like receptor pyrin domain containing 3/cysteine aspartate-specific protease-3 （NLRP3/Caspase-1） pathway proteins in mice with acetaminophen （APAP）-induced acute liver injury. MethodC57BL/6 mice were randomly grouped， and BBD （75， 150， 300 mg·kg-1， ig） was administered twice a day for three days. After 2 hours of the last administration， the mice were treated with APAP （400 mg·kg-1， ip）， and the eyeballs were removed to collect blood after 14 hours. Then they were sacrificed by cervical dislocation for sample collection. Hematoxylin-eosin （HE） staining was used to observe the morphological changes of liver tissue cells， and biochemical methods were used to detect the activities of alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， superoxide dismutase （SOD）， malondialdehyde （MDA） and myeloperoxidase （MPO） in serum of mice in each group. Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） was performed to determine the mRNA expression of tumor necrosis factor-α （TNF-α）， interleukin-1β （IL-1β） and IL-6， and Western blot was performed to determine the protein expression of cyclooxygenase-2 （COX-2）， inducible nitric oxide synthase （iNOS）， NLRP3， Caspase-1 and IL-18 in the liver of mice. ResultCompared with the conditions in normal group， the hepatic lobule structure of mice in the model group was partially destroyed， and the hepatic sinusoids were dilated. And the expression levels of ALT and AST in serum， the protein levels of NLRP3， Caspase-1， iNOS， IL-18 and COX-2 and the mRNA levels of IL-1β， IL-6 and TNF-α were increased （P<0.05， P<0.01）. Compared with the model group， the administration groups had improvement in liver cell rupture and hepatic sinusoidal compression， and a dose-dependent decrease in the levels of ALT and AST in serum as well as the protein levels of NLRP3， Caspase-1， iNOS， IL-18 and COX-2 and the the mRNA levels of IL-1β， IL-6 and TNF-α in liver tissue （P<0.05， P<0.01）. ConclusionBBD can reduce APAP-induced acute liver injury in mice. The mechanism may be related to anti-oxidative stress， inhibition of NLRP3/Caspase-1 pathway， and decreased expression levels of IL-1β， IL-18， TNF-α and IL-6.
