A study on the biological effects of electromagnetic pulse with different high⁃intensity on condylar cartilage in rats
10.12016/j.issn.2096-1456.2023.03.002
- Author:
MA Yuanjun
1
;
WANG Jin
2
;
DONG Qingshan
3
;
HE Feng
4
;
CHEN Xiaohua
4
;
MIAO Hui
4
;
YU Shibin
4
Author Information
1. 1.State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Department of Oral Anatomy and Physiology and TMD, School of Stomatology, Air Force Military Medical University 2Department of Stomatology, Chinese PLA General Hospital of Central Theater Command.
2. Department of Radiation Protection Medicine, Department of Military Preventive Medicine, Air Force Military Medical University
3. Department of Stomatology, Chinese PLA General Hospital of Central Theater Command
4. State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Department of Oral Anatomy and Physiology and TMD, School of Stomatology, Air Force Military Medical University
- Publication Type:Journal Article
- Keywords:
electromagnetic radiation / electromagnetic pulse / temporomandibular joint / condylar / cartilage / cartilage matrix / chondrocytes / apoptosis / matrix metalloproteinase-13
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2023;31(3):162-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the biological effects of electromagnetic pulse (EMP) with different high intensities on condylar cartilage in rats.
Methods:SD rats were randomly divided into a sham group (Sham) and an irradiation group (EMP1: 500 kV/m, 10 Hz; EMP2: 270 kV/m, 10 Hz). Then, they were sacrificed at 1 h, 3 h, 12 h, 24 h and 3 d after irradiation. The degree of cartilage degeneration was evaluated by HE, safranine O-fast green, type Ⅱ collagen immunohistochemistry and TUNEL staining. Immunohistochemistry and western blot were performed to detect the expression of the matrix degradation factors: matrix metalloproteinase-13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS-5) and the apoptosis key factor cleaved-cysteinyl aspartate specific proteinase (cleaved-Caspase3) in condylar cartilage.
Results :HE staining showed that, compared with the Sham group, a small amount of exfoliation was found on the fibrous surface layer of the cartilage after irradiation in the EMP1 and EMP2 groups. Compared with the Sham group, the percentage of safranine O-fast green-positive area decreased significantly at 12 h and 24 h (both P<0.01) in the EMP1 group and 12 h and 24 h in the EMP2 group (both P<0.05); the percentage of type Ⅱ collagen-positive area decreased significantly at 3 h and 12 h (P<0.05, P<0.001) in the EMP1 group. In addition, the number of TUNEL-positive apoptotic cells increased significantly at 1 h, 3 h, 12 h, and 24 h in the EMP1 group and 1 h, 3 h, and 12 h in the EMP2 group (P<0.05). Moreover, at different timepoints (except at 3 d) in the EMP1 group and EMP2 group, the percentage of MMP-13, ADAMTS-5- and cleaved Caspase3-positive chondrocytes and their protein levels in condylar cartilage increased significantly after irradiation (P<0.05).
Conclusion: EMP with a certain degree of high-intensity can induce early transient damage to condylar cartilage. This effect is dose-and time-dependent.
- Full text:不同高强度电磁脉冲对大鼠髁突软骨的生物学效应研究.pdf
