Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage.
- Author:
Kyu Lee HAN
1
;
Jongweon LEE
;
Don Soo KIM
;
Soon Jung PARK
;
Kyung il IM
;
Tai Soon YONG
Author Information
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords: Acanthamoeba culbertsoni; free-living ameba; mouse; DDRT-PCR; virulence
- MeSH: Virulence/genetics; Up-Regulation; Serial Passage; Reverse Transcriptase Polymerase Chain Reaction/methods; Molecular Sequence Data; Mice, Inbred ICR; Mice; Genes, Protozoan/genetics; *Gene Expression Regulation; Gene Expression Profiling/methods; DNA, Protozoan/biosynthesis/*physiology; DNA, Complementary/biosynthesis; Cloning, Molecular/methods; Brain/parasitology; Blotting, Northern/methods; Animals; Amebiasis/mortality/*parasitology; Acanthamoeba/*genetics/*pathogenicity
- From:The Korean Journal of Parasitology 2006;44(1):15-20
- CountryRepublic of Korea
- Language:English
- Abstract: Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from 5% to 70%. Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors.
