In vivo determination of the gap2 gene promoter activity in Giardia lamblia.
- Author:
Hye Won YANG
1
;
Juri KIM
;
Tai Soon YONG
;
Soon Jung PARK
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Giardia lamblia; gap2 gene expression; encystation; luciferase; transfection
- MeSH: Transfection/methods; Time Factors; Recombinant Fusion Proteins/analysis/biosynthesis; Promoter Regions (Genetics)/*physiology; Plasmids; Luciferases/genetics/metabolism; Life Cycle Stages/physiology; Giardia lamblia/*genetics; Genetic Engineering/methods; Genes, Reporter/genetics; Genes, Protozoan/genetics/*physiology; Gene Order; Gene Expression/genetics/*physiology; GTPase-Activating Proteins/*genetics; Blotting, Southern/methods; Animals
- From:The Korean Journal of Parasitology 2006;44(1):21-26
- CountryRepublic of Korea
- Language:English
- Abstract: A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.
