The effect of interleukin-10 on KC gene expression in mouse peritoneal macrophages.
10.12701/yujm.1998.15.1.47
- Author:
Hee Sun KIM
1
Author Information
1. Department of Microbiology, College of Medicine , Yeungnam University, Taegu, Korea.
- Publication Type:Original Article
- Keywords:
KC;
interleukin-10(IL-10)
- MeSH:
Animals;
Cytokines;
Gene Expression*;
Interleukin-10*;
Macrophages;
Macrophages, Peritoneal*;
Mice*;
RNA Stability;
RNA, Messenger
- From:Yeungnam University Journal of Medicine
1998;15(1):47-54
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Interleukin-10(IL-10) inhibits production of a wide range of cytokines in various cell types and transcriptionally inhibits lipopolysaccharide(LPS)-induced expression of proinflammatory mediators. Cytokine expression by macrophages is an important aspect to ochestrate inflammatory responses. As an approach to identify mechanistic targets of IL-10, it was examined the time course for expression of KC(murine homologue of Gro) gene in murine peritoneal macrophages stimulated with LPS with or without IL-10. The effect of IL-10 on LPS induced KC mRNA expression was delayed and only seen after 1 hour treatment. Pretreatment with IL-10 did not eliminate the delayed inhibitory response nor increase the magnitude of suppression. These effects did not depend upon time of IL-10 treatment but the time of LPS treatment. LPS-induced KC mRNA expression by inhibitoy action of IL-10 was not controlled at the level of transcription. The result indicates that IL-10 acts late in the process of KC gene expression and that the prominant site of action may be mRNA stability or translation.
