Comparison of Three Third-generation Anti-HCV Enzyme Immunoassay Tests.
10.12701/yujm.1998.15.1.143
- Author:
Hee Soon CHO
1
;
Jin Young MOON
;
Chae Hoon LEE
;
Kyung Dong KIM
Author Information
1. Department of Clinical Pathology College of Medicine, Yeungnam University Taegu, Korea.
- Publication Type:Original Article
- Keywords:
Hepatitis C virus;
Anti-HCV;
Third-generation Enzyme Immunoassay
- MeSH:
Hepacivirus;
Hepatitis C;
Immunoblotting;
Immunoenzyme Techniques*;
Mass Screening
- From:Yeungnam University Journal of Medicine
1998;15(1):143-150
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The aim of this study was to evaluate domestic enzyme immunoassay(EIA) kit ?LG HCD 3.0?(LG) for the detection of antibody to hepatitis C virus(anti-HCV) in comparision with Axsym HCV version 3.0(Axsym), Cobas Core anti-HCV EIA(Cobas). Cobas kit shows better clear distinction between positive and negative by signal/cutoff ratio(S/C), but it also reveal relatively high false positive rate. The concordance rate of test results between LG and Axsym was 96.2%, between LG and Cobas was 95.5%, and total agreement between three EIA kit was 93.9%. LG were relative poor distinction between positive and negative results, but it could be applied clinically as a screening tool for hepatitis C in general population. The S/C of one false negative result by LG was 0.91, and false positive were less than 4.0, therefore we concluded it is necessary to confirm by immunoblotting assay when S/C were between 0.8 and 4.0.
