Neurobiological Characteristics of Human Histo-amniotic Mesenchymal and its Effect to Treat Parkinson's Disease Modle Mice
- VernacularTitle:人羊膜间充质细胞的神经生物学特性及其治疗帕金森模型小鼠的实验研究
- Author:
Zhe CAI
;
Zhongshu ZHOU
;
Qing XIANG
;
Jingwei HU
;
Lin PAN
;
Lan ZHANG
;
Jun SHU
;
Bo XU
;
Yan LIANG
;
Pingping ZUO
;
Chun HE
;
Yang XU
;
Yan GAO
;
Xiaohui CUI
- Publication Type:Journal Article
- Keywords:
human histio-amniotic mesenchymal (hAMCs), brain transplantation, Parkinson's disease (PD), dopamine
- From:
Chinese Journal of Rehabilitation Theory and Practice
2010;16(4):318-321
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the neurobiological characteristics of human histio-amniotic mesenchymal (hAMCs) and effect of hAMCs transplantation into the brain to treat Parkinson's disease(PD) modle mice.MethodsThe expressions of mesenchymal stem cells, neural stem cells, dopaminergic neurons and markers related to neurogenesis such as Vimentin, STRO-1, nestin, CD133, β-tubulin, TH, DAT, Ngn2 and mash-1 in hAMCs were evaluated through immunocytochemical stain; and the mRNA transcriptions of neural stem cell markers, Vimentin and nestin in hAMCs were detected by RT-PCR. The PD model was induced by MPTP(i.p.) in C57BL/6 mice transplanted with hAMCs into the right striatum. The therapeutical effect of hAMCs on PD mice was evaluated by spontaneous movement, rotating bar test and the immunohistochemistry of anti-human chondrosome and TH antibodies in striatum.ResultshAMCs induced by nerve cells culture medium, expressed mesenchymal stem cells, neural stem cells, dopaminergic neurons and other specific markers related to neurogenesis mentioned above. The frequency of spontaneous movement in PD mice was significantly increased(P<0-05), and the time of rotating bar was obviously prolonged(P<0-05) after transplantation with hAMCs.ConclusionhAMCs possess the characteristics of nerve cells after cultured in vitro and can significantly recover the damage of motor function induced by MPTP after transplantation into striatum in PD model mice.
