Effect of Nasal Administration of Compound of Cholera Toxin B Subunit and Nerve Growth Factor on Spatial Learning and Memory Abilities in the Amnesic Mice
- VernacularTitle:霍乱毒素B亚单位-神经生长因子耦联制剂滴鼻对拟痴呆小鼠空间学习记忆的影响
- Author:
Qing ZHANG
;
Yanyong LIU
;
Limin BAI
;
Geming SHI
;
Xuancai WAN
;
Hongmei SUN
;
Pingping ZUO
- Publication Type:Journal Article
- Keywords:
cholera toxin B subunit, nerve growth factor, β-amyloid protein, Morris water maze, choline acetyl transferase
- From:
Chinese Journal of Rehabilitation Theory and Practice
2010;16(4):311-313
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effects of the conjugate prepared from the cholera toxin B subunit(CB) and nerve growth factor(NGF) on the spatial learning and memory abilities and cholinergic function.MethodsThe conjugate of CB-NGF was prepared by the improved sodium metaperiodate method and nasally administrated to the β-amyloid protein(Aβ25-35) induced amnesic mice for 7 days with 2 dosage (7-5 μg/d、15 μg/d). Spatial learning and memory abilities were evaluated by Morris water maze and cholinergic function was assessed with the choline acetyl transferase (ChAT) immunohistochemical methods.ResultsMorris water maze test showed that the escape latency in Aβ25-35-treated mice prolonged and the staying time reduced in the crossed first quadrant where the platform had been located, compared with the control mice (P<0-01). In addition, the number of ChAT positive neuron declined in the model mice(P<0-001). CB-NGF nasal administration significantly shortened the escape latency and elevated the staying time and number of ChAT positive neuron(P<0-01).ConclusionCB-NGF treatment can improve the spatial and memory performance which may involve the neuroprotection to cholinergic system.
