Study on inhibitory effects of Wubao capsule on airway inflammation in asthmatic model mice by regulating the upstream and downstream cytokines of type Ⅱ innate lymphoid cells
- VernacularTitle:五宝胶囊通过调节Ⅱ型固有淋巴细胞上下游细胞因子抑制哮喘模型小鼠气道炎症的研究
- Author:
Qiushi ZHANG
1
;
Feng HUANG
1
;
Xiaoyun TONG
2
;
Zhuya YANG
3
;
Baojing LI
3
;
Yuping LIN
3
;
Lu QU
3
;
Jian NIE
4
;
Chunyan HU
3
Author Information
1. Yunnan Key Laboratory of Southern Medicinal Utilization，Yunnan University of Chinese Medicine，Kunming 650500，China
2. Dept. of Scientific Research，the First Affiliated Hospital of Yunnan University of Chinese Medicine，Kunming 650021，China
3. School of Chinese Materia Medica，Yunnan University of Chinese Medicine，Kunming 650500，China
4. School of Basic Medical College，Yunnan University of Chinese Medicine，Kunming 650500，China
- Publication Type:Journal Article
- Keywords:
Wubao capsule;
type Ⅱ innate lymphoid cells;
asthma;
airway inflammation
- From:
China Pharmacy
2023;34(2):156-160
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the effects of Wubao capsule on airway inflammation in asthmatic model mice by regulating upstream and downstream cytokines of type Ⅱ innate lymphoid cells （ILC2s）. METHODS Totally 40 female BABL/c mice were randomly divided into normal group， model group， positive control group （dexamethasone 1 mg/kg）， Wubao capsule low-dose and high-dose groups （0.5， 1 g/kg）， with 8 mice in each group. Asthma models were induced by ovalbumin （OVA） sensitization and nebulization. Each group was given normal saline or drug intragastrically for 7 consecutive days. The contents of IgE and OVA-IgE in serum， the contents of interleukin 5 （IL-5）， IL-9， IL-13， IL-25， IL-33， thymic stromal lymphopoietin （TSLP） and mucin 5AC （MUC5AC） in bronchoalveolar lavage fluid （BALF） were determined by ELISA. HE staining was used to observe the pathological changes of lung tissues in mice. PAS staining was used to observe the changes of goblet cell proliferation in each group. The number of ILC2s in lung tissue was determined by flow cytometry （except for Wubao capsule low-dose group）. RESULTS Compared with model group， the contents of IgE and OVA-IgE in serum and the contents of IL-5， IL-9， IL-13， IL-25， IL-33， TSLP and MUC5AC in BALF were significantly reduced in Wubao capsule high-dose and low-dose groups （P＜0.01）. The infiltration of inflammatory cells and the thickening of basement membrane in lung tissue was alleviated to varying degrees， and the proliferation of goblet cells was inhibited； the number of ILC2s in lung tissues of mice in Wubao capsule high-dose group was significantly reduced （P＜0.01）. CONCLUSIONS Wubao capsule could effectively reduce the number of ILC2s in lung tissue， the contents of upstream and downstream cytokines of ILC2s in BALF of asthmatic model mice， so as to inhibit the airway inflammation and improve asthma.
