Research progress of next-generation gene editing tools

VernacularTitle:新一代基因编辑工具研究进展
Author: Rui WANG ¹ ; Xinjie ZHOU ; Xiqin DU ; Di HAO ; Chen WANG ; Bingjie ZOU ; Qinxin SONG ; Guohua ZHOU
Author Information

1. 中国药科大学药物质量与安全预警教育部重点实验室
Publication Type:Journal Article
Keywords: gene editing; CRISPR/Cas9; base editing; prime editing; double-strand break; progress
From: Journal of China Pharmaceutical University 2022;53(6):633-642
CountryChina
Language:Chinese
Abstract: Gene editing tools with nucleases as the main component have now implemented programmable targeted mutagenesis or insertion or deletion of mammalian genomes.From zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), CRISPR/Cas system to safer and more accurate Cas9 fusion protein gene editing tools and other nuclease gene editing tools, this paper systematically describes the development and evolution of gene editing, with detailed introduction to the development and optimization of next-generation gene editing tools, and a prospect of the clinical application of and challenges for gene editing tools.