Glutamate-induced Dynamic Expression of Aquaporin 4 in Cultured Rat Astrocytes
- VernacularTitle:谷氨酸引起培养大鼠星形胶质细胞水通道蛋白4表达动态变化
- Author:
Yang LU
;
Zhongfang SHI
;
Fang YUAN
;
Ming HAN
- Publication Type:Journal Article
- Keywords:
glutamate, aquaporin 4, astrocyte, rat
- From:
Chinese Journal of Rehabilitation Theory and Practice
2010;16(1):29-31
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo determine whether glutamate induces the alteration of aquaporin 4 (AQP4) expression in cultured rat astrocytes. MethodsThe secondary cultured astrocytes were treated with 1 mmol/L L-glutamate for 1 h, 3 h, 6 h, 12 h, 24 h and 48 h. The morphologic changes of astrocytes were observed through microscope after GFAP immunostaining and AQP4 mRNA expression were detected with real-time PCR. ResultsThe astrocytes swelled when exposed to glutamate for 1 h and remained with prolonged treatment. Meanwhile, the AQP4 mRNA expression were early down-regulated and subsequently up-regulated, featured with the lowest AQP4 mRNA level at 12 h after treatment (P<0.01) and higher at 48 h (P<0.05). ConclusionAquaporin 4 may be involved in the occurrence and development of astrocyte swelling induced by glutamate.
