Comparison of Experimental Conditions of CCK-8 and MTS for Human Amniotic Epithelial Cells Proliferation Assay
10.3969/j.issn.1006-9771.2012.09.010
- VernacularTitle:CCK-8 和MTS 法检测人羊膜上皮细胞增殖的比较
- Author:
Yanqiu LIU
;
Kehua ZHANG
;
Yunliang WANG
;
Jun SHU
;
Xue LAI
;
Liqun WU
;
Shanxia CAO
;
Hong LI
;
Yang XU
;
Yan GAO
;
Xiaohui CUI
;
Heming ZUO
;
Zhe CAI
- Publication Type:Journal Article
- Keywords:
MTS, CCK-8, amniotic epithelial cells, cell proliferation, cytotoxicity
- From:
Chinese Journal of Rehabilitation Theory and Practice
2012;18(9):827-830
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the optimal experiment conditions of CCK-8 and MTS for cell proliferation assays in human amniotic epithelial cells and to evaluate the cytotoxicity of these reagents. Methods Human amniotic epithelial cells (hAECs) in logarithm growth stages were prepared in different cell concentrations with DMEM/F12 and 10% FBS. The sensitivity and optimal wavelengths was determined based on the optical density (OD) measured at 450 nm and 492 nm. The optimal time was determined under the conditions of the same cell concentration and defined OD values. HAECs were treated with DMSO, CCK-8 and MTS for 1 h, 2 h, 3 h, and 4 h, respectively. 24 h later, cytotoxicity of the CCK-8 and MTS was evaluated by determination of cell proliferation and Trypan Blue staining. Results The optimal detection wavelength was 450 nm for CCK-8, and 492 nm for MTS. The sensitivity of CCK-8 was slightly lower then that of MTS. The optimal time for incubation hAECs with CCK-8 was 4 h within 1~4 h. The inhibitory on cell proliferation and cytotoxicity of CCK-8 were weaker then those of MTS. Conclusion CCK-8 is a convenient reagent with low cytotoxicity for detection of the proliferation of hAECs.
