Role of active ADM microparticle in skin wound healing of diabetic mice
10.3760/cma.j.issn.1671-0290.2022.03.017
- VernacularTitle:活性脱细胞真皮基质微粒在裸鼠糖尿病创面的作用研究
- Author:
Hao WANG
1
;
Wanling ZHENG
;
Yating YIN
;
Pingping WANG
;
Caiqi SHEN
;
Peisheng JIN
;
Aijun ZHANG
Author Information
1. 徐州市第一人民医院整形美容科，徐州 221000
- Keywords:
Mice, nude;
Stem cells;
Stromal vascular fraction;
Neovascularization;
Adipose-derived stem cells;
Diabetic wound healing
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2022;28(3):231-234
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of adipose-derived stem cells (ADSCs) and ADM microparticle on diabetic wound healing.Methods:ADSCs was co-cultured with ADM microparticle in vitro. The models of diabetic nude mice were established by intraperitoneal injection of STZ and the full-thickness skin defects were designed on the back. All 24 diabetic mice were randomly divided into 4 group: experimental groups were transplanted with ADSCs and ADM microparticle and the other groups were transplanted with ADSCs, ADM microparticle and blank control group was set up. On the 7th and 14 th days, the wound healing rate of 3 mice randomly selected from each group was calculated, and the thickness between dermis and epidermis was measured by hematoxylin and eosin staining. The density of neovascularization was measured by immunohistochemical staining. The differences were compared between the groups.Results:Compared to the ADSCs groups, the mice of the experimental groups showed higher cell survival rate. The wound healing rate in the experimental groups was (86.0±2.7)% (7 days) and (98.5±1.1)% (14 days), thicker dermis-epidermis distance was (99.1±1.8) μm (7 days) and (124.3±4.3) μm (14 days) ( P<0.05), and higher density of neovascularization was noted. Conclusions:The transplantation with active ADM microparticle can significantly promote neovascularization and wound healing of diabetic wound.
