Relationship of pks gene islands with virulence genes and biofilm formation in Klebsiella pneumoniae
10.3760/cma.j.cn112309-20220513-00158
- VernacularTitle:肺炎克雷伯菌中 pks基因岛与毒力基因和生物膜形成的关系
- Author:
Chenshuo LUO
1
;
Yanshu CHEN
;
Yulan LIN
;
Tongzhe DENG
;
Xueni HU
;
Bin YANG
Author Information
1. 福建医科大学附属第一医院检验科，福州 350000
- Keywords:
Klebsiella pneumoniae;
pks gene island;
Virulence;
Biofilm
- From:
Chinese Journal of Microbiology and Immunology
2022;42(11):888-893
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship of pks gene islands with virulence genes, capsular serotypes and biofilm formation in Klebsiella pneumoniae ( Kp). Methods:A total of 113 Kp clinical isolates were collected in the First Affiliated Hospital of Fujian Medical University and divided into two groups based on the presence of pks gene islands: pks+ and pks- groups. The hypermucoviscous (HM) phenotype was detected by string test. Five virulence genes ( peg-344, rmpA, rmpA2, iucA, iroB) and six common capsular serotypes (K1, K2, K5, K20, K54, K57) were detected by polymerase chain reaction (PCR). The biofilm formation ability was measured by crystal violet staining. Results:Among the 113 Kp isolates, 46 were pks+ strains and 67 were pks- strains. The detection rate of HM phenotype was higher in the pks+ group than in the pks- group [87.0% (40/46) vs 43.3% (29/67)]. The detection rates of virulence genes ( peg-344, rmpA, rmpA2, iucA, iroB) and K1 serotype in the pks+ group were also higher than those in the pks- group ( P<0.05). The biofilm formation ability of the pks+ strains was higher than the pks- strains ( P<0.05). Conclusions:Kp strains carrying pks gene islands were likely to display a HM phenotype and mainly belonged to the K1 serotype. Most of the pks+Kp strains carried the virulence genes of peg-344, rmpA, rmpA2, iucA and iroB, and had a greater ability to form biofilms.
