Normalization and parallel comparison of the detection results of antibodies against SARS-CoV-2
10.3760/cma.j.cn112309-20220426-00132
- VernacularTitle:新型冠状病毒抗体检测结果的标准化和平行比较
- Author:
Wenling WANG
1
;
Huijuan WANG
;
Baoying HUANG
;
Yao DENG
;
Li ZHAO
;
Fei YE
;
Wen WANG
;
Jiao REN
;
Wenjie TAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所，国家卫生健康委员会生物安全重点实验室，北京 102206
- Keywords:
SARS-CoV-2;
SARS-CoV-2 IgG antibody;
Neutralizing antibody;
Live virus-based neutralization assay;
Pseudovirus neutralization assay;
ELISA
- From:
Chinese Journal of Microbiology and Immunology
2022;42(10):761-768
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve the consistency of test results through reducing inter-laboratory variation in SARS-CoV-2 antibody detection with WHO SARS-CoV-2 antibody candidate international standard (IS, sample G) and antibody reference panel (samples E, F, H, I, J).Methods:Ten WHO samples (A-J) including the candidate IS and reference panel were evaluated using different methods, such as microneutralization tests based on live SARS-CoV-2, pseudovirus neutralization assay and commercial ELISA kits. The test results were compared using statistical analysis.Results:Using IS (sample G) as a reference, the relative concentrations of other samples could be determined with less variation. ELISA and pseudovirus neutralization assay had consistent results with those obtained with the microneutralization test based on SARS-COV-2 strain HB02. Weakly positive samples could be detected only by a certain kit.Conclusions:The availability of an IS for antibodies would facilitate the standardization of SARS-CoV-2 antibody detection methods. The reference panel fitted all the assays based on the SARS-CoV-2 prototype Wuhan strain. Pseudovirus neutralization assay and ELISA could be used as alternatives to live SARS-CoV-2-based neutralization test to some extent.
