Evaluation of the performance of two commercial SARS-CoV-2 neutralizing antibody test kits
10.3760/cma.j.cn112309-20220302-00069
- VernacularTitle:两种商品化新型冠状病毒中和抗体检测试剂盒的应用性能评价
- Author:
Huijuan WANG
1
;
Yan YANG
;
Baoying HUANG
;
Yao DENG
;
Li ZHAO
;
Wenling WANG
;
Wenjie TAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所，国家卫生健康委员会生物安全重点实验室，北京 102206
- Keywords:
SARS-CoV-2;
Neutralizing antibody;
ELISA;
Live virus micro-neutralization test;
Youden index
- From:
Chinese Journal of Microbiology and Immunology
2022;42(7):535-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the performance of two commercial EIA kits for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies.Methods:Two commercial SARS-CoV-2 neutralizing antibody ELISA test kits (A and B) were used to detect serum panel consists of the following sera: 44 collected before vaccination, 120 collected one month after vaccination and 64 collected six months after recovery from convalescent patients of COVID-19. In the meantime, the above samples were also taken for live virus micro-neutralization test (micro-NT) indicated as the 50% neutralization antibody titer (NT 50). The consistency of qualitative and quantitative results between the two commercial kits and live virus neutralization test was analyzed. Results:Taking the micro-NT results as the standard, the positive coincidence rates of A and B kits were 97.40% and 100.00%, respectively; the negative coincidence rates were 97.30% and 95.95%, respectively; the Youden indices were 0.95 and 0.96, respectively. Furthermore, quantitative analysis indicated that the correlation coefficients between A and B kits and micro-NT results were 0.24 ( P<0.05) and 0.52 ( P<0.000 1) for samples collected after vaccination, respectively; while the correlation coefficients were 0.81 ( P<0.000 1) and 0.89 ( P<0.000 1) for convalescent serum samples, respectively. Conclusions:The results obtained by the two commercial neutralizing antibody detection kits were in good agreement with the qualitative results of micro-NT. The neutralizing antibody titers in convalescent serum samples detected by the two kits showed a stronger correlation with the micro-NT results.
