Transcranial magnetic stimulation at low frequency can promote the recovery of neurological functioning after a stroke
10.3760/cma.j.issn.0254-1424.2022.07.001
- VernacularTitle:重复经颅磁刺激对缺血性脑卒中小鼠神经功能障碍及NLRP3表达的影响
- Author:
Yushu JIANG
1
;
Wei LI
;
Lingzhi QIN
;
Milan ZHANG
;
Xiaojuan WANG
;
Rui PANG
;
Huiqin LIU
;
Jiewen ZHANG
Author Information
1. 河南省人民医院（郑州大学人民医院）神经内科，郑州 450003
- Keywords:
NOD-like receptor family pyrin domain containing 3;
Repetitive transcranial magnetic stimulation;
Pyroptosis;
Ischemic stroke;
Neurological dysfunction;
In
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2022;44(7):577-582
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore any effect of repeated transcranial magnetic stimulation (rTMS) on the recovery of neurological functioning and the expression of NOD-like receptor family pyrin domain containing 3 (NLRP3) and inflammatory factors after ischemic stroke.Methods:Sixty-four C57BL/6J mice were randomly divided into a normal control group, a model group, a sham stimulation group and an observation group, each of 16. All mice except those of the normal control group received middle cerebral artery occlusion using the suture method to model an ischemic stroke. After the modeling the observation group was given 1Hz rTMS daily for 7 consecutive days, while the sham stimulation group was given sham rTMS. After the intervention, Zea-Longa scores were used for all of the groups, and the size of the cerebral infarct was measured using triphenyltetrazolium chloride staining. The expression of NLRP3 around the cerebral infarction was detected using immunofluorescence, while that in the brain tissue was measured using Western blotting. The expression of interleukin-1β and IL-18 in the brain tissue was detected using enzyme-linked immunosorbent assays.Results:Compared with the normal control group, a significant increase was observed in the other groups′ average neurological function impairment scores. Expression of NLRP3, IL-1β and IL-18 in the model and sham stimulation groups also increased, with large cerebral infarcts in the cortex and hippocampus. Compared with the sham stimulation and model groups, there was a significant decrease in the average neurological dysfunction scores, the area of cerebral infarction in the cortex and hippocampus, as well as the expression of NLRP3, IL-1β and IL-18 in the observation group.Conclusions:Low-frequency rTMS can promote the recovery of damaged nerve function after an ischemic stroke, at least in mice. It can reduce the size of cerebral infarction, and inhibit neuronal pyroptosis, which is closely related to the down-regulation of NLRP3, IL-1β and IL-18 expression.
