Protective Effects of the Green Tea Polyphenol(-)-epigallocatechin Gallate Against Ischemia Reperfusion Injury Induced by Middle Cerebral Artery Occlusion in Rats.
- Author:
Young Bin CHOI
1
;
Jeong Wook PARK
;
Si Ryung HAN
;
Kwang Soo LEE
;
Beum Saeng KIM
Author Information
1. Department of Neurology, College of Medicine, The Catholic University of Korea, Korea. neurotec@hanmail.net
- Publication Type:Original Article
- Keywords:
EGCG;
Ischemia;
Rat
- MeSH:
Animals;
Brain;
Brain Injuries;
Brain Ischemia;
Humans;
Infarction, Middle Cerebral Artery*;
Ischemia*;
Ketamine;
Lipid Peroxidation;
Male;
Middle Cerebral Artery*;
Models, Animal;
Neuroprotective Agents;
Nylons;
Rats;
Rats, Wistar;
Reperfusion Injury*;
Sutures;
Tea*;
Xylazine
- From:Journal of the Korean Neurological Association
2003;21(4):387-391
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: EGCG(epigallocatechin gallate), a major green tea extract, is a potent free radical scavenger which has been shown to reduce free radical-induced lipid peroxidation. The purpose of this study was to examine whether EGCG reduces focal ischemia/reperfusion-induced brain injury in rats. METHODS: Male Wistar rats were anesthetized with ketamine and xylazine and subjected to 120 min of temporary middle cerebral artery occlusion by an intraluminal nylon suture coated with poly-L-lysine. The drug (EGCG, n=8) or vehicle (normal saline, n=8) was administered iv.(as a 50 mg/kg bolus) immediately after the onset of middle cerebral artery occlusion. Neurologic status was evaluated 2 hours after occlusion and 24 hours after. Twenty-four hours after ischemia, the brain was perfusion-fixated and the infarct volume was determined. RESULTS: EGCG significantly improved the neurological status at 24 hours after middle cerebral artery occlusion.(p<0.05), and reduced total infarct volumes (p<0.01). CONCLUSIONS: These results demonstrate the neuroprotective effect of EGCG in a rat model of transient focal cerebral ischemia.
