The study of effect of Human macrophage polarization on Mouse Pericyte-to-Myofibroblast Transition in vitro
10.3760/cma.j.cn421203-20220210-00018
- VernacularTitle:人巨噬细胞极化对小鼠周细胞-肌成纤维细胞转分化的体外作用研究
- Author:
Zijie WANG
1
;
Zeping GUI
;
Ming ZHENG
;
Zhou HANG
;
Zhijian HAN
;
Jun TAO
;
Xiaobin JU
;
Ruoyun TAN
;
Min GU
Author Information
1. 南京医科大学第一附属医院泌尿外科，南京 210029
- Keywords:
Kidney transplantation;
Macrophage;
Chronic graft dysfunction
- From:
Chinese Journal of Organ Transplantation
2022;43(6):346-351
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of macrophage polarization on pericyte-to-myofibroblast transition and renal allograft fibrosis after kidney transplantation(KT).Methods:Allograft tissues were harvestedfrom recipients with chronic allograft dysfunction(CGD)and normal kidney tissues.The expression and distribution of M1/M2 macrophages in kidney tissues were detected by routine and immunofluorescent staining; mRNA of CD68, CD206 and iNOS detected by polymerase chain reaction(PCR); Murine vascular pericytes subjected to TGF-β1 in vitro and the expressions of α-SMA and PDGFR-β in perivascular cells detected by immunoblotting and cellular fluorescence; The co-culturing models of vascular pericytes and M1/M2 macrophages were constructed.The expressions of α-SMA and PDGFR-β in pericytes were detected by immunoblotting, cellular fluorescence and PCR.Results:A marked infiltration of CD68+ iNOS+ M1 macrophages was present in allograft tissues of recipients with CGD while no obvious infiltration of CD68 + CD206 + was observed.The mRNA levels of CD68, iNOS and CD206 were significantly higher in CGD group than those in control group( P<0.05); In CGD allograft tissues, protein expressions of α-SMA and PDGFR-β spiked markedly( P<0.05)while cells with double staining of α-SMA and PDGFR-β were markedly infiltrated in interstitial area of CGD allograft.TGF-β1 could induce a marked elevation of PMT-related markers in a time-dependent manner( P<0.05); Immunoblotting and cellukar fluorescence indicated that M1 macrophages could promote the elevations of α-SMA and PDGFR-β in pericytes in vitro while M2 macrophages showed no effect on pericyte-to-myofibroblast transition in pericytes. Conclusions:M1 macrophage polarization may promote the formation of renal allograft interstitial fibrosis through promoting PMT.
