Correlation between post-transplant non-HLA antibodies and humoral rejection after kidney transplantation
10.3760/cma.j.cn421203-20220117-00010
- VernacularTitle:肾移植术后非HLA抗体与体液性排斥反应相关性研究
- Author:
Shaoyong ZHUANG
1
;
Ruoyang CHEN
;
Dawei LI
;
Haoyu WU
;
Jiajin WU
;
Junbo HE
;
Ming ZHANG
;
Xiaodong YUAN
Author Information
1. 上海交通大学医学院附属仁济医院泌尿外科，上海 200120
- Keywords:
Kidney transplantation;
Glutathione S-transferase;
Humoral rejection
- From:
Chinese Journal of Organ Transplantation
2022;43(6):328-333
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the correlation between post-transplant non-HLA antibodies and humoral rejection(HR)after kidney transplantation(KT).Methods:A retrospective study was conducted for KT recipients with non-HLA antibody level detected from September 2019 to January 2021.The recipients with biopsy confirmed HR and donor-specific HLA antibodies negative or feeble positive at the time of HR were designated as HR group while recipients with stable renal allograft function from 2 weeks post-KT to the time of detecting non-HLA antibody as stable group.The levels of HLA antibody, MHC classⅠchain-related gene A(MICA)antibody and 32 non-HLA antibodies were tested by Luminex single antigen bead and the levels of angiotensin Ⅱ type 1 receptor(AT1R)antibody quantified by enzyme-linked immunosorbent assay (ELISA). Inter-group differences in positive rate of non-HLA antibodies and number of positive non-HLA antibodies were analyzed.Results:Twenty-four recipients had positive non-HLA antibodies while the remainders had no positive non-HLA antibodies.Three HR recipients were positive for actin antibody, collagen Ⅲ antibody, glutathione S-transferase theta-1 antibody or IFN-γ antibody respectively.However, all four non-HLA antibodies of stable recipients were negative.There was significant inter-group difference( P=0.017). Four HR recipients were positive for collagenⅡantibody while only 1 stable recipient was positive for collagenⅡantibody.The positive rate of collagenⅡ antibody was significantly higher in HR recipients than that in stable recipients( P=0.023). HR recipients had an average of 2.36 positive non-HLA antibodies while stable recipients had an average of 0.90.There was significant inter-group difference ( P=0.008). Conclusions:A high level of non-HLA antibodies may elevate the risk of HR after KT.
