RASAL3 promotes the proliferation of intrahepatic cholangiocarcinoma cells by up regulating the expression of FXYD6
10.3760/cma.j.cn113855-20220110-00017
- VernacularTitle:RASAL3通过上调FXYD6表达促进肝内胆管细胞癌细胞增殖的作用机制研究
- Author:
Bin ZHANG
1
;
Dachen ZHOU
;
Zhongbiao CHEN
;
Weichen WANG
;
Hui HOU
;
Qiru XIONG
;
Guoqiang PING
Author Information
1. 安徽医科大学第二附属医院普外科五病区，合肥 230601
- Keywords:
Bile ducts,intrahepatic;
Cholangiocarcinoma;
RASAL3;
FXYD6;
Na +-K +-ATPase
- From:
Chinese Journal of General Surgery
2022;37(11):839-844
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of RASAL3 in intrahepatic cholangiocarcinoma (iCCA) and the mechanism of promoting iCCA development.Methods:Tumor and paracancerous tissues were collected from 185 iCCA patients, the expression of RASAL3 was detected by immunohistochemistry, RT-qPCR and Western blot. The expression of RASAL3 and FXYD6 mRNA and protein in human cholangiocarcinoma cell line and human bile duct epithelial cells were detected with RT-qPCR and Western blot, the cell proliferation was detected with CCK-8 assay, and the activity of Na +-K +-ATPase was also detected. Results:RASAL3 was highly expressed in cholangiocarcinoma tissues and cell lines; Survival analysis showed that RASAL3 overexpression was associated with poor prognosis of cholangiocarcinoma( P<0.05) and knockdown of RASAL3 inhibits the proliferation of cholangiocarcinoma cells; Silencing RASAL3 decreases the expression of FXYD6 inhibiting the activity of Na +-K +-ATPase. Conclusion:RASAL3 is up-regulated in human cholangiocarcinoma, which can promote the occurrence and development of cholangiocarcinoma by activating FXYD6 and affecting Na +-K +-ATPase activity.
