Effect of ultrasound combined with 4-hydroxyphenyl-retinamide lipid microbubbles on type Ⅰ collagen α1 chain expression in keloid-derived fibroblasts
10.35541/cjd.20200765
- VernacularTitle:超声联合4-羟苯基维胺微泡对瘢痕疙瘩成纤维细胞Ⅰ型胶原蛋白α1链表达的影响
- Author:
Zhouna LI
1
;
Wenyan JIN
;
Zhehu JIN
Author Information
1. 延边大学附属医院皮肤科，延吉 133000
- Keywords:
Keloid;
Collagen type I;
Tretinoin;
Sonication;
4-Hydroxyphenyl-retinamide;
COL1A1;
Microbubble
- From:
Chinese Journal of Dermatology
2022;55(7):596-598
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of ultrasound combined with 4-hydroxyphenyl-retinamide (4-HPR) lipid microbubbles on type Ⅰ collagen α1 chain (COL1A1) protein expression in keloid-derived fibroblasts.Methods:In vitro cultured keloid-derived fibroblasts were divided into 3 groups: control group receiving conventional culture with incomplete Dulbecco′s modified Eagle′s medium (DMEM) , 4-HPR lipid microbubble group cultured with incomplete DMEM containing 15 mg/L 4-HPR lipid microbubbles, and ultrasound + 4-HPR lipid microbubble group cultured with incomplete DMEM containing 15 mg/L 4-HPR lipid microbubbles under ultrasound treatment. After 24-hour treatment, reverse transcription (RT) -PCR and Western blot analysis were performed to determine the mRNA and protein expression of COL1A1 in keloid-derived fibroblasts in each group. Intergroup comparison was carried out by using t test. Results:The mRNA relative expression level of COL1A1 was 1.00 ± 0.18, 0.69 ± 0.15 and 0.35 ± 0.18 in the control group, 4-HPR lipid microbubble group and ultrasound + 4-HPR lipid microbubble group respectively, and the protein relative expression level of COL1A1 was 0.93 ± 0.03, 0.74 ± 0.07 and 0.44 ± 0.06 in the above 3 groups respectively. Moreover, the mRNA and protein expression of COL1A1 was significantly lower in the 4-HPR lipid microbubble group and ultrasound + 4-HPR lipid microbubble group than in the control group ( P < 0.05 or 0.001) , and lower in the ultrasound + 4-HPR lipid microbubble group than in the 4-HPR lipid microbubble group ( P < 0.05) . Conclusion:Ultrasound combined with 4-HPR lipid microbubbles could markedly inhibit the mRNA and protein expression of COL1A1 in keloid-derived fibroblasts.
