Identification of key genes for neuropathic pain in rats
10.3760/cma.j.cn131073.20220227.00612
- VernacularTitle:大鼠神经病理性痛关键基因的筛选
- Author:
Hua LIN
1
;
Chengcheng SONG
;
Chao QIN
;
Keliang XIE
;
Guolin WANG
;
Yonghao YU
Author Information
1. 天津医科大学总医院麻醉科，天津 300041
- Keywords:
Neuropathic pain;
Genes;
Computational biology
- From:
Chinese Journal of Anesthesiology
2022;42(6):704-707
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the key genes for neuropathic pain in rats.Methods:The genomic data of spinal cord tissues of rats (GSE18803) were downloaded from the Gene Expression Database at the American Center for Biotechnology Information to identify differentially expressed genes associated with neuropathic pain, and key genes were obtained by further analysis of the protein-protein interaction networks.Single-cell localization and expression of the key genes were analyzed by the Tabula Muris database.Results:The protein-protein interaction networks identified 10 hub genes, including Tyrobp, Clec4a3, C1qc, Ptprc, Laptm5, Csf1r, C1qa, C1qb, Fcgr3a, Cd53. Cd53, Laptm5 and Ptprc were mainly expressed in macrophages, B cells, NK cells, monocytes and granulocytes. Clec4a3 and Csf1r were mainly expressed in monocytes, Fcgr3a in monocytes and granulocytes, and Tyrobp in macrophages, monocytes, granulocytes, and pluripotent progenitor cells. Conclusions:Ten target genes associated with neuropathic pain are identified using bioinformatics, and their distribution and expression in immune inflammatory cells are obtained through comprehensive analysis.
