Development of a mouse model of stellate ganglion block and subsequent effects on cerebral cortical blood flow
10.3760/cma.j.cn131073.20211129.00411
- VernacularTitle:小鼠星状神经节阻滞模型的建立及其对脑皮质血流量的影响
- Author:
Jiahua WANG
1
;
Wei ZHOU
;
Xiaohong WANG
;
Shiting YAN
;
Shunping TIAN
;
Ying WANG
;
Le-Yang YU
;
Hu LI
;
Dongsheng ZHANG
;
Zhuan ZHANG
;
Weili LIU
Author Information
1. 扬州大学附属医院麻醉科，扬州 225012
- Keywords:
Stellate ganglion;
Nerve block;
Models, animal;
Cerebrovascular circulation;
Cerebral cortex
- From:
Chinese Journal of Anesthesiology
2022;42(4):430-434
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a model of stellate ganglion block (SGB) in mice and investigate the effect of SGB on cerebral cortical blood flow.Methods:Thirty clean-grade healthy male C57BL/6 mice, aged 8-9 weeks, weighing 23-27 g, were divided into 5 groups ( n=6 each) using a random number table method: control group (group C), left SGB group (group L), left normal saline group (group SL), right SGB group (group R) and right normal saline group (group SR). Group C received no intervention.SGB was performed with 0.25% ropivacaine 0.08 ml via percutaneous posterior approach in L and R groups, while the equal volume of normal saline 0.08 ml was given instead at the location of left and right stellate ganglion in SL and SR groups, respectively.The cerebral cortical blood flow was determined using laser speckle contrast imaging system before SGB (T 0) and at 10, 30, 60, 90 and 120 min after SGB (T 1-5). Results:Mice developed ptosis on the block side, indicating that the model of SGB was successfully developed in L and R groups.There was no significant difference in cerebral cortical blood flow at each time point among C, SL and SR groups ( P>0.05), and cerebral cortical blood flow on the block side decreased at T 1, began to increase at T 2, peaked at T 3, and decreased at T 5 which was still higher than that at T 0 in group L and group R ( P<0.01). Compared with C and SL groups, the left cerebral cortical blood flow was significantly decreased at T 1, 5 and increased at T 2-4 in group L ( P<0.01). Compared with C and SR groups, the right cerebral cortical blood flow was significantly decreased at T 1, 5 and increased at T 2-4 in group R ( P<0.01). There were no significant differences in cerebral cortical blood flow at each time point between group C and group SL and between group C and group RL ( P>0.05). Conclusions:The mouse model of SGB via percutaneous posterior approach is successfully developed.Unilateral SGB can affect cerebral cortical blood flow on the block side, which shows a transitory decrease followed by a sustained significant increase.
