Comparison of cell growth suppression in SiHa cervical carcinoma cell line by human papillomavirus type 16 E6/E7 siRNAs.
10.5468/kjog.2010.53.1.35
- Author:
Sae Hyun PARK
1
;
Byung Joon PARK
;
Yong Wook KIM
;
Duck Yeong RO
;
Tae Eung KIM
;
Jae Keun JUNG
;
Su Mi BAE
;
Woong Shick AHN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul, Korea. ahnlab1@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Cervical cancer;
Human papillomavirus;
E6;
E7;
siRNA
- MeSH:
Cell Count;
Cell Line;
Humans;
Oncogenes;
RNA, Messenger;
RNA, Small Interfering;
Transfection;
Uterine Cervical Neoplasms
- From:Korean Journal of Obstetrics and Gynecology
2010;53(1):35-42
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Human cervical cancer is caused by the high-risk types of human papillomavirus (HPV) such as HPV16, which possess the E6 and E7 oncogenes, whose expressions are a prerequisite for cancer development. We performed this study to compare the efficacy of antitumor activity by HPV siRNA which silences only E6 or both E6/E7. METHODS: We transfected siRNA 377 (HPV16 E6 siRNA), siRNA 3 (HPV16 E6 siRNA), and siRNA 198 (HPV16 E7 siRNA) into SiHa cell line (siRNA 377 silences only E6, and siRNA 3 and siRNA 198 silence both E6 and E7). We experimented cell counts and morphologic changes 24 and 48 hours after transfection and expressions of HPV16 E6/E7 mRNA by RT-PCR. RESULTS: siRNA 377, siRNA 3, and siRNA 198 suppressed the cell growth. siRNA 3 and siRNA 198 were more potent than siRNA 377 in cell growth suppression. siRNA 377 knocked down the expression of E6 mRNA, and both siRNA 3 and siRNA 198 knocked down the expression of E6/E7 mRNA. CONCLUSION: Our results suggest that simultaneous suppression of E6 and E7 was more potent than E6-specific suppression in cancer cell growth.
