The role of lncRNA H19 in prognostic evaluation and regulating the radioresistance in colon cancer
10.3760/cma.j.cn113030-20211019-00367
- VernacularTitle:lncRNA H19对结肠癌预后评估及辐射抗性的调控作用研究
- Author:
Linying LI
1
;
Jiangtao WANG
;
Wei CHENG
;
Qiang LI
;
Xiaodong JIN
Author Information
1. 中国科学院近代物理研究所/中国科学院重离子束辐射生物医学重点实验室/甘肃省重离子束辐射医学应用基础重点实验室，兰州 730000
- Keywords:
Colonic neoplasms;
Radioresistance;
LncRNA H19;
Prognosis;
Cell cycle
- From:
Chinese Journal of Radiation Oncology
2022;31(12):1153-1159
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of lncRNA H19 in evaluating prognosis and regulating radioresistance of colon cancer, aiming to provide a new potential target for the diagnosis and treatment of colon cancer.Methods:The value of lncRNA H19 in the clinicopathological parameters and prognosis of colon cancer was assessed based on bioinformatics technology. The expression of lncRNA H19 in HCT116 and SW620 cells was regulated through siRNA and overexpression plasmid transfection, respectively. The effect of regulating lncRNA H19 expression on the proliferation, DNA synthesis, radiosensitivity and cell cycle of colon cancer cells after X-ray irradiation were detected by CCK8, EdU, cell clonogenic survival assay and flow cytometry.Results:The expression of lncRNA H19 was significantly upregulated in colon cancer tissues and correlated with poor prognosis in colon cancer patients. LncRNA H19, as a high-risk gene for colon cancer, had a significant advantage for prognostic assessment of colon cancer (AUC=0.816). Furthermore, the expression of lncRNA H19 was upregulated after X-ray irradiation in colon cancer cells. Knockdown of lncRNA H19 (siRNA-H19) significantly increased the radiosensitivity in HCT116 cells, while overexpression of lncRNA H19 (H19-OE) enhanced the radioresistance in SW620 cells. Moreover, flow cytometry revealed that the G 2/M phase arrest induced by X-ray irradiation was obviously aggravated after siRNA-H19 treatment in colon cancer cells, which suggested that lncRNA H19 might regulate the radiosensitivity by inhibiting cell cycle progression. Conclusion:LncRNA H19 plays a key role in the prognostic assessment and regulating the radiosensitivity in colon cancer, which can be used as a potential target for improving radiosensitivity of colon cancer radiotherapy.
