Effects of Halothane Anesthesia and Surgical Procedure on Human Growth Hormone , Insulin and Glucose Level in Plasma .
10.4097/kjae.1978.11.4.361
- Author:
Jung Hun PARK
1
Author Information
1. Department of Anesthesiology, College of Medicine, Chosun University, Kwangju, Korea.
- Publication Type:Original Article
- MeSH:
Anesthesia*;
Atropine;
Blood Glucose;
Blood Pressure;
Body Weight;
Diazepam;
Female;
Glucose*;
Growth Hormone;
Halothane*;
Heart Rate;
Human Growth Hormone*;
Humans*;
Insulin;
Male;
Metabolism;
Methods;
Oxygen;
Plasma*;
Premedication;
Radioimmunoassay;
Recovery Room;
Reference Values;
Reflex;
Ventilation
- From:Korean Journal of Anesthesiology
1978;11(4):361-370
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was undertaken to investigate the effects of halothane anesthesia alone on blood glucose metabolism in ten surgical patients by determining plasma growth hormone ,(HGH), insulin and glucose, and to compare these with the effects of halothane anesthesia plus surgery. Each patient was premedicated with diazepam, 5 mg and atropine sulfate, 0. 5mg intramuscu Jarly one hour before the induction of halothane anesthesia. After endotracheal intubatien had been carried out following SCC 30-40mg, anesthesia was mairrtained with halothane and oxygen. The muscle relaxant (gallamine) in divided doses was injected during surgery when needed and ventilation was controlled or assisted intermittently throughout the procedure. A moderate depth of anesthesia was maintained according to clinical judgement, based on signs including blood pressure, pulse rate and somatie reflex responses to the surgical xtimulation. Eight blood samples were obtained from each patient:( I ) at 09.00 a.m. immediately before :induction of anesthesia (this sample served as acoatrol value) (2) the next three were after 15. 30 and 45 minutes of halothane anesthesia but before the start of the operation; (3) further three, 15,30 and 45 minutes, after the start of the operation;(4) in the recovery room after fully awaking. HGH RIAKIT was utilized as a kit for determination of human growth hormoae by radioimmunoassay and also lNSULIN-RIAKlT for insulin levels in the blood, For the determination of blood glucose level, OTB method was utilized. The ratio between male and female pgtients was 5:5, mean age 39. 2+/-12. 1 and average body weights 55. 5+/-8 .5 kg. The mean control plasma growth hormone level(HGH) in 10 surgical patients after premedication, and immediately before induction of anesthesia was 2. 03+/-0. 28ng/ml. The mean yre-surgical concentration of HGH in plasma after 15, 30 and 45 minutes of halothane anesthesia rose to 2.63+/-0.41 ng/ml, 2.76+/-0.35 ng/ml, and 2.95+/-0.42 ng/ml, respectively but these values were not significantly elevated above the control value. The plasma levols significantly increased to 3. 42+/-0. 5 mg/ml (P <0. 05), 6. 64+/-0. 6 mg/ml (p <0.001) and 7.83+/-0,75 ng/ml. (p<0.001) 15 minutes, 30 minutes and 45 minutes respectively after the start of the operation. It decreased to 3. 58+/-0. 68 ng/ml. (p<0. 05) in the recovery room when the patient awoke fully. The mean pre-anesthetic control insulin level in plasma. in 10 surgical patients was 12. 6+/-2. 0 ng/ml which was within the normal range. This level. did not vary appreciably during halothane anesthesia alone, during operation or in the post-operative period. The mean control pre-anesthetic blood glucose level was 78. .7+/-3.9 mg/100ml. It rose to 80. 6+/-2. 9 mg/100ml. 83. 2+/-3. 6mg/100 ml 30minutes, 45minutes and of halothane anesthesia alone and 15 minutes of the start of the operation but these changes were not significant. It increased significanty to 98. 2+/-4. 4 mg/100ml(p<0. 05) and 103. 9+/-4. 0 mg/100ml (p<0. 001) 30 minutes and 45 minutes respectively after the start of the surgery but it rose to 95.7+/-3.1mg/ 100ml (p <0. 01) in the post-operative period. Consequently the plasma HGH level during halothane anesthesia alone for 45 minutes was slightly, increased and rose significantly during operation and in the post-operative period. The peak level was achieved 45 minutes after the start of surgery. Plasma insulin levels did not change appreciably during anesthesia alone or during surgery. Blood glucose levels increased slightly during anesthesia alone but rose significantly during operation and in the post-operative period.
