Establishment and identification of C57BL/6 mouse model with radiation-induced pulmonary fibrosis
10.3760/cma.j.cn113030-20211215-00520
- VernacularTitle:小鼠放射性肺纤维化模型的建立与检测
- Author:
Meng YUAN
1
;
Yu MEN
;
Xin SUN
;
Maoyuan ZHAO
;
Dan BAO
;
Xu YANG
;
Shuang SUN
;
Yongxing BAO
;
Zeliang MA
;
Yunsong LIU
;
Zhouguang HUI
Author Information
1. 国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院放疗科，北京 100021
- Keywords:
Radiation-induced pulmonary fibrosis;
C57BL / 6 mouse;
Animal model
- From:
Chinese Journal of Radiation Oncology
2022;31(10):928-932
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the mouse model with radiation-induced pulmonary fibrosis, and to identify and analyze it from the aspects of function, imaging and pathology.Methods:Thirty C57BL/6 mice were randomly divided into the control group, 16 Gy irradiation group and 20Gy irradiation group. The mice in the irradiation groups received a single 16 Gy or 20 Gy chest X-ray irradiation, and underwent functional examination, imaging examination and pathological examination at 3 and 6 months after irradiation.Results:At 6 months after irradiation, hair on the chest and back of the mice turned white and fell off, and the airway resistance was increased significantly. CT images showed extensive patch shadows and consolidation in the lung. Three dimensional reconstruction suggested that the lung of mice was distorted and deformed, and the volume was decreased significantly. Pathological examination confirmed that there was extensive pulmonary fibrosis.Conclusions:Significant pulmonary fibrosis occurs after 6 months of chest irradiation in mice. The animal model of radiation-induced pulmonary fibrosis in C57BL/6 mice was successfully established.
