Mechanism of Sedum alfredii extract alleviating radiation damage in human small intestinal epithelial cells HIEC-6
10.3760/cma.j.cn112271-20220104-00002
- VernacularTitle:东南景天提取物减缓人小肠上皮细胞HIEC-6辐射损伤机制的初步研究
- Author:
Tong ZHU
1
;
Junbo HE
;
Xin WU
;
Zhouxuan WANG
;
Saijun FAN
Author Information
1. 中国医学科学院北京协和医学院放射医学研究所 天津市放射医学与分子核医学重点实验室，天津 300192
- Keywords:
Sedum alfredi extract;
Radiation damage;
PRKN;
Endoplasmic reticulum;
ROS
- From:
Chinese Journal of Radiological Medicine and Protection
2022;42(7):493-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To confirm the mechanism of Sedum alfredii extract (SafE) alleviating radiation injury in human small intestinal epithelial cells (HIEC-6). Methods:HIEC-6 cells were divided into 4 groups, including control group (Con), irradiation group (IR), SafE alone group (SafE) and SafE plus irradiation group (SafE+ IR). All of the SafE groups were treated with 0.02 g/ml (W/V) SafE for 24 h. Cell viability (CCK-8 method ) and intracellular ROS levels were investigated at 24 h after 2, 4, and 6 Gy irradiation. Samples were taken at 24 h after 4 Gy irradiation for transcriptome analysis, and the intracellular E3 ubiquitin ligase PRKN expression level was measured. The thickness of endoplasmic reticulum was detected at 24 h after 4 Gy irradiation using fluorescent dye.Results:SafE could maintain cell viability after irradiation ( t=2.94-10.40, P<0.05), and significantly reduced the level of ROS in the irradiated cells ( t=-13.29--4.53, P<0.05). PRKN was preliminarily verified to be the target gene of SafE that maintained PRKN transcript level and endoplasmic reticulum thickness after irradiation (IR group vs. Con group: t=-5.55, 3.27, P<0.05, SafE group vs. SafE+ IR group: P>0.05). Conclusion:SafE is effective in maintaining ER thickness and reducing cellular radiation damage and its target gene PRKN could be regulated by ionizing radiation.
