The proliferation promoting effect of eIF2α on fibroblasts of rheumatoid arthritis synovium
10.3760/cma.j.cn141217-20220216-00059
- VernacularTitle:真核翻译起始因子2α促进类风湿关节炎滑膜中成纤维细胞增殖的研究
- Author:
Aidong LIU
1
;
Xueli ZHAO
;
Guangyi XIONG
;
Hui TANG
Author Information
1. 天津市天津医院（天津大学天津医院）病理科，天津 300211
- Keywords:
Arthritis, rheumatoid;
Synovium;
Fibroblasts;
Eukaryotic translation initiation factor 2α;
Proliferation
- From:
Chinese Journal of Rheumatology
2022;26(9):590-595,F3
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expression characteristics of eukaryotic translation initiation factor 2α(eIF2α), and analyze its proliferation regulation effect on fibroblasts of rheumatoid arthritis synovium.Methods:The synovial tissues were collected in patients with rheumatoid arthritis(RA)(40 cases) and osteoarthritis(OA)(40 cases). EIF2α and proliferating cell nuclear antigen(PCNA) were detected by immunohistochemistry method. Fibroblast cell line of rheumatoid arthritis synovium(MH7A) were cultured to establish si-eIF2α group(siRNA-eIF2α plasmid transfection), vector transfection group and blank control group in vitro. PCNA was detected by Western blot method, cell proliferation activity was detected by CCK-8 method. χ2 test was performed on count data, two-sample t-test was performed on quantitative data, one-way analysis of variance (ANOVA) was performed to compare the means of more than two groups, regression equation was calculated by correlation regression analysis. Results:The positive rate of eIF2α was significantly higher in RA synovial fibroblasts than that of OA [52.5%(21/4) vs 20.00%(8/20), χ2=9.14, P=0.003]. Positive correlation was found between eIF2α and PCNA in RA ( Y=0.366 X+2.220, P=0.001) . Compared with blank control group and vector transfection group, cell proliferation activity decreased significantly in si-eIF2α group of MH7A cell line at 72 h [(0.65±0.08) vs (0.96±0.12) vs (1.09±0.06), F=4.52, P=0.022] and 96 h [(1.13±0.14) vs (1.42±0.97) vs (1.56±0.12), F=9.87, P=0.001) , PCNA expression decreased significantly [(0.84±0.15) vs (1.32±0.18) vs (1.28±0.14), F=5.22, P=0.012) . Conclusion:High expression of eIF2α can promote the proliferation of fibroblasts of RA synovium.
