Peroxisome Proliferator-Activated Receptor-Gamma Expression in the Lung Tissue of Obese Rats.
10.3349/ymj.2011.52.3.495
- Author:
Su Jin HWANG
1
;
Jung Ho KIM
;
Jae Won SHIM
;
Duk Soo KIM
;
Hye Lim JUNG
;
Moon Soo PARK
;
Won Young LEE
;
Se Yeon KIM
;
Jung Yeon SHIM
Author Information
1. Department of Pediatrics, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea. jy7.shim@samsung.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Obesity;
peroxisome proliferator activated receptor;
adiponectin receptor;
lung;
leptin;
TNF-alpha
- MeSH:
Animals;
Body Weight;
Glucose Tolerance Test;
Leptin/genetics/metabolism;
Lung/*metabolism;
Male;
Obesity/genetics/*metabolism;
PPAR gamma/genetics/*metabolism;
RNA, Messenger/metabolism;
Rats;
Rats, Long-Evans;
Receptors, Adiponectin/genetics/metabolism;
Tumor Necrosis Factor-alpha/genetics/metabolism
- From:Yonsei Medical Journal
2011;52(3):495-501
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Obesity is a risk factor for asthma and type II diabetes. Peroxisome proliferator-activated receptor (PPAR)-gamma has been suggested to regulate inflammatory responses in diabetes and asthma. We investigated whether PPAR-alpha, PPAR-gamma, adiponectin receptors (AdipoR1, AdipoR2), leptin, and tumor necrosis factor (TNF)-alpha are expressed in rat lung tissues and whether the expression differs between obese Otsuka Long-Evans Tokushima Fatty (OLETF) and lean Long Evans Tokushima Otsuka (LETO) rats. MATERIALS AND METHODS: Obese and lean rats were given with a high fat diet or a 30% restricted diet for 32 weeks, and their blood glucose levels and weights were monitored. After 32 weeks, mRNA levels of PPAR-alpha, PPAR-gamma, AdipoR1, AdipoR2, leptin, and TNF-alpha in lung tissues were measured using real time PCR. RESULTS: PPAR-alpha, PPAR-gamma, AdipoR1, AdipoR2, leptin, and TNF-alpha were expressed in both obese and lean rat lung tissues. Increased serum glucose levels on intraperitoneal glucose tolerance testing and a higher weight gain at 32 weeks were observed in OLETF control rats compared to OLETF diet restricted rats. PPAR-gamma expression was markedly elevated in obese control and diet restricted rats compared to lean rats, although PPAR-gamma expression in obese rats was not affected by diet restriction. Leptin was highly expressed in OLETF rats compared to LETO rats. TNF-alpha expression was enhanced in OLETF control rats compared LETO diet restricted rats, and decreased by diet restriction. PPAR-alpha, AdipoR1, and AdipoR2 expression were not significantly different between obese and lean rats. CONCLUSION: PPAR-gamma was highly expressed in the lung tissues of obese rats and may be a novel treatment target for regulating lung inflammation associated with obesity.
